AB11256

Anti-Vimentin antibody

Name: Anti-Vimentin antibody (ab11256) | Abcam
Brand: Abcam Limited
SKU: AB11256
Price: 400 USD
Availability: InStock
Rating: 2 (4 reviews)

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What is this?

(4 Reviews)

(39 Publications)

Goat Polyclonal Vimentin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 39 publications.

View Alternative Names

Vimentin, VIM

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB11256)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB11256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of 0.1% trypsin treated human skin tissue labelling Vimentin with ab11256 at 1/20 dilution. FITC-conjugated rabbit anti-goat IgG (whole molecule) was used as the secondary antibody (1/20 dilution).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Specificity

No reactivity was observed with the other families of intermediate filaments, including, desmin, keratin, neurofilaments and glial filaments.The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20", "IHCP-species-notes": "<p></p>" } } }

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Product details

If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Purification notes

The antiserum has been treated to remove lipoproteins.

Storage buffer

Preservative: 0.097% Sodium azide Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

See full target information VIM

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1632245 PubMed40772038

2025

Complementary detection strategies for circulating tumor cells in breast cancer: clinical implications of combining immunofluorescence and cytopathological staining.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Jesenko,Cvetka Grasic Kuhar,Ziva Pisljar,Simona Miceska,Veronika Kloboves-Prevodnik,Maja Cemazar

Iranian journal of basic medical sciences 27:1574-1582 PubMed39539438

2024

Baicalein blocked gastric cancer cell proliferation and invasion through modulated platelet type 12-lipoxygenase.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ye,Dan Qiao,Yingying Zhang,Yingshi Piao,Jingchun Jin

Virchows Archiv : an international journal of pathology 486:1049-1059 PubMed39271482

2024

Aberrant localization of β1 integrin in podocyte cytoplasm of primary FSGS with cellular lesion.

Applications

Unspecified application

Species

Unspecified reactive species

Eisuke Katafuchi,Satoshi Hisano,Satoko Kurata,Kumiko Muta,Noriko Uesugi,Tetsu Miyamoto,Yoshikazu Harada,Shohei Shimajiri,Ritsuko Katafuchi,Toshiyuki Nakayama

Journal of cellular and molecular medicine 28:e18488 PubMed39031896

2024

MCM8 promotes lung cancer progression through upregulating DNAJC10.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Cao,Hongsheng Liu,Zhijun Han,Cheng Huang,Chao Guo,Luo Zhao,Chao Gao,Yuan Xu,Guige Wang,Zhe Feng,Shanqing Li

Nature communications 15:557 PubMed38228638

2024

Enhancing aortic valve drug delivery with PAR2-targeting magnetic nano-cargoes for calcification alleviation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyong Chen,Tanchen Ren,Lan Xie,Haochang Hu,Xu Li,Miribani Maitusong,Xuhao Zhou,Wangxing Hu,Dilin Xu,Yi Qian,Si Cheng,Kaixiang Yu,Jian An Wang,Xianbao Liu

Cancers 15: PubMed36900263

2023

Hedgehog-GLI and Notch Pathways Sustain Chemoresistance and Invasiveness in Colorectal Cancer and Their Inhibition Restores Chemotherapy Efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Citarella,Giuseppina Catanzaro,Zein Mersini Besharat,Sofia Trocchianesi,Federica Barbagallo,Giorgio Gosti,Marco Leonetti,Annamaria Di Fiore,Lucia Coppola,Tanja Milena Autilio,Zaira Spinello,Alessandra Vacca,Enrico De Smaele,Mary Anna Venneri,Elisabetta Ferretti,Laura Masuelli,Agnese Po

Frontiers in cell and developmental biology 10:1001453 PubMed36438566

2022

Immunosuppressants Tacrolimus and Sirolimus revert the cardiac antifibrotic properties of p38-MAPK inhibition in 3D-multicellular human iPSC-heart organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tian,Yuta Tsujisaka,Vanessa Y Li,Kanae Tani,Antonio Lucena-Cacace,Yoshinori Yoshida

Journal of lipid research 63:100294 PubMed36206855

2022

Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Kang Sun,Wang-Sheng Wang

Annals of translational medicine 9:1571 PubMed34790777

2021

Hsa_circRNA_0045861 promotes renal injury in ureteropelvic junction obstruction via the microRNA-181d-5p/sirtuin 1 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Fan,Xiaoming Yin,Qi Zhao,Yi Yang

Journal of visualized experiments : JoVE : PubMed34605811

2021

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yonatan R Lewis-Israeli,Brett D Volmert,Mitchell A Gabalski,Amanda R Huang,Aitor Aguirre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vimentin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB11256)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (39)

Product promise