Anti-Vimentin antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded human lung carcinoma tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB137321)

Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin using ab137321 (green) at a 1/500 dilution at RT for 15 min. Alpha Tubulin, a cytoskeleton marker were labelled with an anti-alpha tubilin antibody (red) at a 1/1000 dilution. The nuclear counter stain is DAPI (blue).

• IP

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Immunoprecipitation - Anti-Vimentin antibody (AB137321)

Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of ab13732. Western blot analysis was performed using ab13732. An anti-Rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-Vimentin antibody (ab137321)

All lanes:

HeLa whole cell extracts

Secondary

All lanes:

anti-Rabbit IgG

Predicted band size: 54 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded adult mouse retina tissue stained for Vimentin with ab137321 (1/250 dilution) (green) in immunohistochemical analysis.

Red : beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded mouse ovary tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded rat testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded mouse colon tissue stained for Vimentin protein at cytoplasm with ab137321 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded mouse testis tissue stained for Vimentin protein at cytoplasm with ab137321 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded rat ovary tissue stained for Vimentin protein at cell membrane and cytoplasm with ab137321 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded mouse intestine tissue stained for Vimentin protein at cytoplasm with ab137321 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• WB

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Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/50000 dilution

Lane 1:

U87-MG cells at 30 µg

Lane 2:

SK-N-SH cells at 30 µg

Lane 3:

IMR32 cells at 30 µg

Lane 4:

SK-N-AS cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/10000 dilution

Lane 1:

Non-transfected 293T cells at 10 µg

Lane 2:

Transfected 293T cells at 10 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/50000 dilution

Lane 1:

Wild-type 293T cells at 30 µg

Lane 2:

Vimentin knockout (KO) 293T cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

10% SDS-PAGE gel

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/50000 dilution

Lane 1:

U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 30 µg

Lane 2:

SK-N-SH (Human neuroblastoma cell line) whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 53 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/5000 dilution

Lane 1:

MCF-7 cells at 30 µg

Lane 2:

MDA-MB-231 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/10000 dilution

Lane 1:

293T whole cell lysate at 20 µg

Lane 2:

293T whole cell lysate at 10 µg

Lane 3:

293T whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 1 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/5000 dilution

Lane 1:

Neuro2A cells at 30 µg

Lane 2:

Rat2 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/5000 dilution

Lane 1:

Neuro2A cells at 30 µg

Lane 2:

NIH-3T3 cells at 30 µg

Lane 3:

Raw264.7 cells at 30 µg

Lane 4:

C2C12 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/10000 dilution

Lane 1:

PC-12 cells at 30 µg

Lane 2:

Rat2 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB137321)

Immunofluorescence analysis of 4% paraformaldehyde fixed MDCK cells labelling Vimentin protein at cytoskeleton using ab137321 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is Hoechst 33342 (Blue).
Scale bar= 10 µm.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (AB137321)

Paraffin-embedded dog pancreas tissue stained for Vimentin protein with ab137321 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-Vimentin antibody (AB137321)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-Vimentin antibody (ab137321) at 1/20000 dilution

Lane 1:

MDCK cells at 30 µg

Lane 2:

PG-4 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 54 kDa

false