Anti-Vimentin rabbit polyclonal antibody that is used to detect Vimentin in Western blot, IHC, ICC/IF, IP. Suitable for Human, Mouse, Rat and other species.
- Recombinant fragment protein within human VIM as immunogen
- Recombinant fragment protein within human VIM as immunogen
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- Cells 12:2023Unraveling Transcriptome Profile, Epigenetic Dynamics, and Morphological Changes in Psoriasis-like Keratinocytes: "Insights into Similarity with Psoriatic Lesional Epidermis".Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmeneh Ghaffarinia,Szilárd Póliska,Ferhan Ayaydin,Aniko Goblos,Shahram Parvaneh,Máté Manczinger,Fanni Balogh,Lilla Erdei,Zoltán Veréb,Kornélia Szabó,Zsuzsanna Bata-Csörgő,Lajos KeményPubMed 38132145
- Cancer cell international 23:2602023Exosomal circRNA RHOT1 promotes breast cancer progression by targeting miR-204-5p/ PRMT5 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeihua Jiang,YinPing Yu,Jianghua Ou,Yongtao Li,Ning ZhuPubMed 37924099
- Oncology reports 50:2023PRMT1 accelerates cell proliferation, migration, and tumor growth by upregulating ZEB1/H4R3me2as in thyroid carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoli Feng,Changju Chen,Yi LuoPubMed 37859611
- Molecular biology reports 50:9757-97672023MicroRNA-499-5p inhibits transforming growth factor-β1-induced Smad2 signaling pathway and suppresses fibroblast proliferation and collagen synthesis in rat by targeting TGFβ-R1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Zhao,Wentao Yang,Xiangdong Li,Hongtao Yuan,Jianping Guo,Yutang Wang,Zhaoliang ShanPubMed 37676431
- Heliyon 9:e195442023miR-708-3p promotes gastric cancer progression through downregulating ETNK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJincai Shang,Qingdong Wang,Jingren Wang,Bo Xu,Shuang LiuPubMed 37809692
- Functional & integrative genomics 23:2812023MiR-22-3p suppresses NSCLC cell migration and EMT via targeting RAC1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuejiao Wang,Xiaobin Wang,Tao Jiang,Zhipei Zhang,Nianlin Xie,Guang YangPubMed 37620594
- Environmental toxicology 38:2002-20102023TRIM29 facilitates proliferation and malignancy of cholangiocarcinoma cells by activating MAPK and β-catenin pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Yang,Wenqing Bao,Haiyan Diao,Shaofei Yang,Lin Qiu,Chunjing Xu,Bin ZhaoPubMed 37219039
- Aging 15:3295-33302023Proteostatic modulation in brain aging without associated Alzheimer's disease-and age-related neuropathological changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPol Andrés-Benito,Ignacio Íñigo-Marco,Marta Brullas,Margarita Carmona,José Antonio Del Rio,Joaquín Fernández-Irigoyen,Enrique Santamaría,Mónica Povedano,Isidro FerrerPubMed 37179123
- Genome integrity 14:22023Therapeutic Fractional Doses of Ionizing Radiation Promote Epithelial-Mesenchymal Transition, Enhanced Invasiveness, and Altered Glycosylation in MCF-7 Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaheem Al-Abedi,Seda Tuncay Cagatay,Ammar Mayah,Susan A Brooks,Munira KadhimPubMed 38025522
- STAR protocols 4:1021642023Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina von der Bey,Silvia De Cicco,Susanne Zach,Bastian Hengerer,Ebru Ercan-HerbstPubMed 36933222
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human VIM. The exact immunogen used to generate this antibody is proprietary information. Database link P08670
Consider this alternative
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Tested |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Expected | Tested | Tested | Tested |
| Guinea pig | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Guinea pig, Cow, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Dog | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Guinea pig, Cow, Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species Dog | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Guinea pig, Cow, Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species Dog | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Guinea pig, Cow, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Alternative names
Vimentin, VIM
Recommended products
Anti-Vimentin rabbit polyclonal antibody that is used to detect Vimentin in Western blot, IHC, ICC/IF, IP. Suitable for Human, Mouse, Rat and other species.
- Recombinant fragment protein within human VIM as immunogen
- Recombinant fragment protein within human VIM as immunogen
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human VIM. The exact immunogen used to generate this antibody is proprietary information. Database link P08670
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Vimentin antibody (ab137321) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, IP, WB in chicken, chinese hamster, cow, dog, human, mouse, rat samples.
Anti-Vimentin antibody (ab137321) has been cited over 257 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Vimentin antibody (ab137321) has high sensitivity and specificity.
Anti-Vimentin antibody (ab137321) specifically detects Vimentin protein (UniProt ID: P08670; Molecular weight: 54kDa) and is sold in 50 µL selling sizes.
Vimentin is a type of intermediate filament protein that plays a significant role in cancer biology. It is commonly used as a marker for epithelial-mesenchymal transition (EMT), a process where epithelial cells acquire mesenchymal properties, which is crucial for cancer metastasis. Vimentin is often overexpressed in various types of cancers and its overexpression is associated with increased tumor growth, invasion, and poor prognosis.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
18 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Paraffin-embedded human lung carcinoma tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Vimentin antibody (ab137321)
10% SDS-PAGE gel
All lanes: Western blot - Anti-Vimentin antibody (ab137321) at 1/50000 dilution
Lane 1: U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts at 30 µg
Lane 2: SK-N-SH (Human neuroblastoma cell line) whole cell extracts at 30 µg
Lane 3: IMR32 whole cell extracts at 30 µg
Lane 4: SK-N-AS whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Paraffin-embedded adult mouse retina tissue stained for Vimentin with ab137321 (1/250 dilution) (green) in immunohistochemical analysis.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Immunoprecipitation - Anti-Vimentin antibody (ab137321)
Vimentin was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 5 μg of ab137321.
Lane 1: HeLa whole cell extracts (Input).
Lane 2: Control IgG in HeLa whole cell extracts.
Lane 3: Vimentin IP in HeLa whole cell extracts.
All lanes: Immunoprecipitation - Anti-Vimentin antibody (ab137321)
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Paraffin-embedded rat testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Paraffin-embedded mouse testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Paraffin-embedded mouse ovary tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab137321 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Immunohistochemical analysis of paraffin-embedded, formalin-fixed RT2 rat xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
Western blot - Anti-Vimentin antibody (ab137321)
7.5% SDS PAGE
All lanes: Western blot - Anti-Vimentin antibody (ab137321) at 1/3000 dilution
All lanes: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 103 kDa, 53 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)
Confocal immunofluorescence analysis of methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin, using ab137321 (green) at a 1/500 dilution. Alpha-tubulin filaments were labelled with an anti-alpha tubilin antibody (red) at a 1/2000 dilution.
Western blot - Anti-Vimentin antibody (ab137321)
10% SDS PAGE
All lanes: Western blot - Anti-Vimentin antibody (ab137321) at 1/3000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Predicted band size: 53 kDa
Western blot - Anti-Vimentin antibody (ab137321)
10% SDS Page
All lanes: Western blot - Anti-Vimentin antibody (ab137321) at 1/1000 dilution
All lanes: NIH-3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 53 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)
Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin using ab137321 (green) at a 1/500 dilution at RT for 15 min. Alpha Tubulin, a cytoskeleton marker were labelled with an anti-alpha tubilin antibody (red) at a 1/1000 dilution. The nuclear counter stain is DAPI (blue).
Immunoprecipitation - Anti-Vimentin antibody (ab137321)
Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of ab13732. Western blot analysis was performed using ab13732. An anti-Rabbit IgG was used as a secondary reagent.
All lanes: Immunoprecipitation - Anti-Vimentin antibody (ab137321)
All lanes: HeLa whole cell extracts
SecondaryAll lanes: anti-Rabbit IgG
Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody (ab137321)
Paraffin-embedded dog pancreas tissue stained for Vimentin protein with ab137321 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab137321)
Immunofluorescence analysis of 4% paraformaldehyde fixed MDCK cells labelling Vimentin protein at cytoskeleton using ab137321 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is Hoechst 33342 (Blue).
Scale bar= 10 µm.
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