Rabbit Polyclonal Vimentin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.
Preservative: 0.07% Sodium azide
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Chimpanzee | Predicted | Predicted |
Cynomolgus monkey | Predicted | Predicted |
Mammals | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 | Notes - |
Species Mouse | Dilution info 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Mammals, Cynomolgus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chimpanzee, Mammals, Cynomolgus monkey | Dilution info - | Notes - |
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Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.
Vimentin, VIM
Rabbit Polyclonal Vimentin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.
Preservative: 0.07% Sodium azide
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.
All lanes: Western blot - Anti-Vimentin antibody (ab188499) at 1/5000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Predicted band size: 53 kDa
All lanes: Western blot - Anti-Vimentin antibody (ab188499) at 1/10000 dilution
All lanes: HeLa cell extract
Developed using the ECL technique.
Predicted band size: 53 kDa
Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.
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