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Rabbit Polyclonal Vimentin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499), expandable thumbnail
  • Western blot - Anti-Vimentin antibody (AB188499), expandable thumbnail
  • Western blot - Anti-Vimentin antibody (AB188499), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.07% Sodium azide

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human VIM. Database link P08670

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Chimpanzee
Predicted
Predicted
Cynomolgus monkey
Predicted
Predicted
Mammals
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

-

Species
Mouse
Dilution info
1/10000
Notes

-

Predicted
Predicted

Species
Chimpanzee, Mammals, Cynomolgus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Chimpanzee, Mammals, Cynomolgus monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Rabbit Polyclonal Vimentin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human VIM. Database link P08670
Purity
Whole antiserum
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.

  • Western blot - Anti-Vimentin antibody (ab188499), expandable thumbnail

    Western blot - Anti-Vimentin antibody (ab188499)

    All lanes: Western blot - Anti-Vimentin antibody (ab188499) at 1/5000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lane 2: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

    Lane 3: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

    Lane 4: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

    Predicted band size: 53 kDa

  • Western blot - Anti-Vimentin antibody (ab188499), expandable thumbnail

    Western blot - Anti-Vimentin antibody (ab188499)

    All lanes: Western blot - Anti-Vimentin antibody (ab188499) at 1/10000 dilution

    All lanes: HeLa cell extract

    Developed using the ECL technique.

    Predicted band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (ab188499)

    Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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