AB188499

Anti-Vimentin antibody

Lab Essentials
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(5 Publications)

Rabbit Polyclonal Vimentin antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VIM.

View Alternative Names

Vimentin, VIM

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

Immunofluorescent analysis of HeLa cells labeling Vimentin with ab188499 at 1/5000 dilution (red). Counter stained with a monoclonal antibody to Lamin A + C (green). DNA is stained blue.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab188499 at 1/5000 dilution in ICC/IF. Counterstained with mouse mAb to Actin at 1/500 dilution (red). Blue is DAPI staining of nuclear DNA.

Supplier Data

Western blot - Anti-Vimentin antibody (AB188499)

All lanes:

Western blot - Anti-Vimentin antibody (ab188499) at 1/5000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 4:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Predicted band size: 53 kDa

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Supplier Data

Western blot - Anti-Vimentin antibody (AB188499)

All lanes:

Western blot - Anti-Vimentin antibody (ab188499) at 1/10000 dilution

All lanes:

HeLa cell extract

Predicted band size: 53 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VIM.

P08670

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.07% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

See full target information VIM

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0087823 PubMed37905840

2023

Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Pahmeier,Teresa-Maria Lavacca,Sarah Goellner,Christopher J Neufeldt,Vibhu Prasad,Berati Cerikan,Sreejith Rajasekharan,Giulia Mizzon,Uta Haselmann,Charlotta Funaya,Pietro Scaturro,Mirko Cortese,Ralf Bartenschlager

Cell host & microbe 28:853-866.e5 PubMed33245857

2020

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Cortese,Ji-Young Lee,Berati Cerikan,Christopher J Neufeldt,Viola M J Oorschot,Sebastian Köhrer,Julian Hennies,Nicole L Schieber,Paolo Ronchi,Giulia Mizzon,Inés Romero-Brey,Rachel Santarella-Mellwig,Martin Schorb,Mandy Boermel,Karel Mocaer,Marianne S Beckwith,Rachel M Templin,Viktoriia Gross,Constantin Pape,Christian Tischer,Jamie Frankish,Natalie K Horvat,Vibor Laketa,Megan Stanifer,Steeve Boulant,Alessia Ruggieri,Laurent Chatel-Chaix,Yannick Schwab,Ralf Bartenschlager

The Journal of pharmacology and experimental thera 370:416-426 PubMed31248980

2019

Platelet-Specific Deletion of Cyclooxygenase-1 Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Sacco,Annalisa Bruno,Annalisa Contursi,Melania Dovizio,Stefania Tacconelli,Emanuela Ricciotti,Paloma Guillem-Llobat,Tania Salvatore,Luigia Di Francesco,Rosa Fullone,Patrizia Ballerini,Vincenzo Arena,Sara Alberti,Guizhu Liu,Yanjun Gong,Alessandro Sgambato,Carlo Patrono,Garret A FitzGerald,Ying Yu,Paola Patrignani

Molecular medicine reports 18:2753-2761 PubMed30015869

2018

miR-577 suppresses cell proliferation and epithelial-mesenchymal transition by regulating the WNT2B mediated Wnt/β-catenin pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Liwei Sun,Jinduo Li,Rong Jiang

Oncology research 26:529-536 PubMed28653599

2017

[ARTICLE WITHDRAWN] MicroRNA-539 Inhibits the Epithelial-Mesenchymal Transition of Esophageal Cancer Cells by Twist-Related Protein 1-Mediated Modulation of Melanoma-Associated Antigen A4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhili Cao,Xiang Zheng,Lei Cao,Naixin Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vimentin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody (AB188499)

Western blot - Anti-Vimentin antibody (AB188499)

Western blot - Anti-Vimentin antibody (AB188499)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise