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Chicken Recombinant Monoclonal Vimentin antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (AB323247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (AB323247), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (AB323247), expandable thumbnail

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Human
Tested
Tested
Mouse
Expected
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity).Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Alternative names

Chicken Recombinant Monoclonal Vimentin antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype

IgY

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3776

Purification technique

Affinity purification Thiophilic Resin

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

ab323247 is the carrier-free version of Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) ab323238.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Vimentin also known by its scientific name VIM is a type of intermediate filament protein that forms part of the cytoskeleton. It has a molecular mass of approximately 57 kDa. Vimentin is expressed in various cell types including mesenchymal cells fibroblasts and endothelial cells. This protein maintains cell integrity and provides resistance against stress by organizing cellular architecture and anchoring organelles. Researchers often use antibodies such as anti-vimentin or anti-mouse vimentin to study its expression and functions in cell biology.

Biological function summary

The role of vimentin involves providing structural support and participating in processes like cell division and wound healing. Vimentin forms complexes with other cytoskeletal proteins significantly impacting cell shape and mobility. Its involvement in cellular stability becomes paramount during events requiring cellular reorganization such as embryogenesis and inflammation. These functions are frequently observed using labeled antibodies like Alexa Fluor 568 and Alexa Fluor 647 for fluorescent imaging.

Pathways

Vimentin plays a role in the epithelial-to-mesenchymal transition (EMT) and interacts within the TGF-beta signaling pathway. It works alongside proteins like N-cadherin and fibronectin in the EMT pathway contributing to increased cell motility and survival. This involvement positions vimentin as a control point reflecting changes in cell phenotypes under physiological and pathological conditions.

Associated diseases and disorders

Vimentin has relevance in cancer metastasis and cardiovascular diseases. Its aberrant expression correlates with tumor progression and metastatic potential by affecting cell movement and invasion. Vimentin also associates with proteins like collagen and integrins in the context of cardiovascular pathologies influencing vascular stiffness and remodelling. By targeting vimentin researchers aim to understand intervention strategies that may mitigate these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (ab323247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (ab323247)

    Immunofluorescence staining of VIM in sections of formalin-fixed paraffin-embedded mouse ovary (positive) and mouse skeletal muscle (negative).

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) ab323238 at 1/500 dilution and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    This data was developed using the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (ab323247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (ab323247)

    Immunofluorescence staining of VIM in sections of formalin-fixed paraffin-embedded human kidney (positive) and human skeletal muscle (negative)*.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) ab323238 at 1/500 dilution and then incubated for 1 hour with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173 Goat Anti-Chicken IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    This data was developed using the same antibody clone in a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (ab323247), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) – BSA and Azide Free (ab323247)

    Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) ab323238 staining VIM in Hap1 (positive) and Hap1-VIM KO (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker – Chicken IgY (Chimeric) ab323238 at 1ug/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed ab150173, Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This data was developed using the same antibody clone in a different buffer formulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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