Name: Anti-Vimentin antibody [VI-10]
SKU: ab20346
Rating: 5 (21 reviews)

Anti-Vimentin antibody [VI-10] (ab20346) is a mouse monoclonal antibody that is used to detect Vimentin in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Chicken, Human, Mouse, Rat samples.

- Specificity confirmed with Vimentin knockout cell line validation

Images

Western blot - Anti-Vimentin antibody [VI-10] (AB20346), expandable thumbnail

Publications

Key facts

Isotype: IgM
Host species: Mouse
Storage buffer: pH: 8
Preservative: 0.097% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	Flow Cyt	WB	IHC-P
Human	Tested	Expected	Tested	Tested
Mouse	Expected	Tested	Expected	Expected
Rat	Tested	Expected	Expected	Expected
Chicken	Tested	Expected	Expected	Expected
Pig	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1 µg/mL	Notes Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
Species Chicken	Dilution info 1 µg/mL	Notes Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.
Species Human	Dilution info 1 µg/mL	Notes Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1 µg for 10⁶ Cells	Notes Mouse IgM [B11/7] - Isotype control ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Chicken, Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Chicken, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1.00000-5.00000 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Chicken, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Pig	Dilution info -	Notes -

Associated Products

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Target data

See full target information VIM

Function

Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Vimentin, VIM

Key facts

Isotype: IgM
Host species: Mouse
Storage buffer: pH: 8
Preservative: 0.097% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: VI-10
Purification technique: Proprietary technique
Light chain type: unknown
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
Lanes 1 - 2: Merged signal (red and green). Green - ab20346 observed at 57 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

ab20346 was shown to specifically react with Vimentin in wild-type HAP1 cells as signal was lost in VIM (Vimentin) knockout cells. Wild-type and VIM (Vimentin) knockout samples were subjected to SDS-PAGE. ab20346 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Vimentin antibody [VI-10] (ab20346) at 1 µg/mL
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: VIM (Vimentin) knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 53 kDa
Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
Image from Sato M et al., J Clin Med., 8, 695. Fig 3.; doi: 10.3390/jcm8050695.
Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
ab20346 staining Vimentin in wild-type HAP1 cells (top panel) and Vimentin knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab20346 at 1μg/ml dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Image from Gao MQ et al, J Cell Sci. 2010 Oct 15;123(Pt 20):3507-14. Epub 2010 Sep 14, Fig 3. DOI 10.1242/jcs.072900
Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
ab20346 used at a 1/1000 dilution in Western Blot.5-20μg of total protein from each sample was loaded.
All lanes: Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
Predicted band size: 53 kDa
Flow Cytometry - Anti-Vimentin antibody [VI-10] (ab20346)
Overlay histogram showing NIH3T3 cells stained with ab20346 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20346, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was a Goat Anti-Mouse DyLight® 488 (IgG; H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (Mouse IgM [B11/7] - Isotype control ab91545, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NIH3T3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Vimentin with ab20346 at 5 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)
Human normal skin. Staining is localised to cytoplasm. Left panel: with primary antibody at 1 ug/mL. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-10] (ab20346)
Immunofluorescence staining of RBL rat basophiilioc cell line with anti-Vimentin (VI--10). Nuclei are stained with DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-10] (ab20346)
Immunocytochemical analysis of chicken postnatal erythrocytes labeling Vimentin with ab20346 at 1 ug/ml (green). Cells were counterstained with anti-alpha-tubulin (red) and DAPI (blue).
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
Vimentin western blot using anti-Vimentin antibody [VI-10] ab20346. Publication image and figure legend from Shen, E., Wang, X., et al., 2020, Cancer Cell Int, PubMed 32190000.

ab20346 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab20346 please see the product overview.
Repressed miR-93-5p inhibits HGC-27 cell migration, invasion and EMT. a The migration and invasion ability of GC cells determined by Transwell assay (×200). Blank group: HGC-27 cells without transfection of any plasmids; Inhibitor NC group: HGC-27 cells transfected with NC of miR-93-5p; miR-93-5p inhibitor group: HGC-27 cells transfected with miR-93-5p inhibitor. b, c Migration and invasive ability of GC cells determined by the Transwell assays and the quantitative analysis. d Morphological characteristics of HGC-27 cells after transfection with inhibitor NC or miR-93-5p inhibitor (×100). e Immunofluorescence to determine the expression of EMT-related markers (E-cadherin, Vimentin and Snail) (×400). f Western blot assay and quantitative analysis for the expression of AHNAK, E-cadherin, Vimentin and Snail. Data were measurement data and expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multi-groups. The cell experiment was repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001