Rabbit Recombinant Monoclonal Vimentin phospho S39 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.
Images
![Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s39-antibody-ep1069y-ab52942--western-blot-img44337.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)")
![Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s39-antibody-ep1069y-ab52942--dot-blot-img4984.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)")
Publications
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- Frontiers in bioengineering and biotechnology 10:7354652022Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Guo,Yajie Chang,Jingjie Li,Chuanchuan Zhou,Rui Huang,Xing Yang,Guihua Liu,Xiaoyan LiangPubMed 35547156
- JID innovations : skin science from molecules to population health 2:1000762022A Series of BRAF- and NRAS-Driven Murine Melanoma Cell Lines with Inducible Gene Modulation Capabilities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlah Bok,Ariana Angarita,Stephen M Douglass,Ashani T Weeraratna,Florian A KarrethPubMed 35146482
- Science signaling 14:eabc85792021Systems approach reveals distinct and shared signaling networks of the four PGE receptors in T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna M Lone,Piero Giansanti,Marthe Jøntvedt Jørgensen,Enio Gjerga,Aurelien Dugourd,Arjen Scholten,Julio Saez-Rodriguez,Albert J R Heck,Kjetil TaskénPubMed 34609894
- Aging 13:957-9722020Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Liu et. al.PubMed 33290258
- Oncology letters 12:4051-40532016Classical intracranial chondrosarcoma: A case report.
- Nature communications 5:44282014Filamin acts as a key regulator in epithelial defence against transformed cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMihoko Kajita et. al.PubMed 25079702
- PloS one 8:e739002013Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells.
- Journal of biotechnology 145:111-92009Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnssi J Mähönen,Kaisa-Emilia Makkonen,Johanna P Laakkonen,Teemu O Ihalainen,Sari P Kukkonen,Minna U Kaikkonen,Maija Vihinen-Ranta,Seppo Ylä-Herttuala,Kari J AirennePubMed 19903502
- American journal of physiology. Cell physiology 298:C221-362009Filamin A is required for vimentin-mediated cell adhesion and spreading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHugh Kim,Fumihiko Nakamura,Wilson Lee,Yulia Shifrin,Pamela Arora,Christopher A McCullochPubMed 19776392
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/200000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Vimentin
Recommended products
Rabbit Recombinant Monoclonal Vimentin phospho S39 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1069Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s39-antibody-ep1069y-ab52942--western-blot-img44337.jpg "Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)")
Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)
Blocking and dilution buffer: 5% NFDM/TBST.All lanes: Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942) at 1/1000000 dilution
Lane 1: HeLa whole cell lysate - untreated at 10 µg
Lane 2: HeLa whole cell lysate - treated with Calyculin A at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa
Exposure time: 3min
![Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s39-antibody-ep1069y-ab52942--dot-blot-img4984.jpg "Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)")
Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)
Dot blot analysis of Vimentin (pS39) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S39) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s39-antibody-ep1069y-ab52942--western-blot-img136596.gif "Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)")
Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)
All lanes: Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942) at 1/200000 dilution
Lane 1: HeLa cell lysate untreated at 10 µg
Lane 2: HeLa cell lysate Calyculin A treated at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa
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