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Rabbit Recombinant Monoclonal Vimentin phospho S39 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytDotWBIHC-PIHC-Fr
Human
Not recommended
Not recommended
Expected
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Predicted
Predicted
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Predicted
Predicted
Not recommended
Not recommended
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/200000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Synthetic peptide

Dilution info

-

Notes

-

Target data

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Alternative names

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Rabbit Recombinant Monoclonal Vimentin phospho S39 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 9 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1069Y

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942), expandable thumbnail

    Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942) at 1/1000000 dilution

    Lane 1: HeLa whole cell lysate - untreated at 10 µg

    Lane 2: HeLa whole cell lysate - treated with Calyculin A at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 53 kDa

    Observed band size: 54 kDa

    Exposure time: 3min

  • Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942), expandable thumbnail

    Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)

    Dot blot analysis of Vimentin (pS39) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S39) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942), expandable thumbnail

    Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942)

    All lanes: Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942) at 1/200000 dilution

    Lane 1: HeLa cell lysate untreated at 10 µg

    Lane 2: HeLa cell lysate Calyculin A treated at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 53 kDa

    Observed band size: 54 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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