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AB52942

Anti-Vimentin (phospho S39) antibody [EP1069Y]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

3

(1 Review)

|

(12 Publications)

Rabbit Recombinant Monoclonal Vimentin phospho S39 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 12 publications.

View Alternative Names

Vimentin

3 Images
Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)
  • WB

Unknown

Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)

All lanes:

Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942) at 1/200000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate Calyculin A treated at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 54 kDa

false

Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)
  • WB

Lab

Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (ab52942) at 1/1000000 dilution

Lane 1:

HeLa whole cell lysate - untreated at 10 µg

Lane 2:

HeLa whole cell lysate - treated with Calyculin A at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 53 kDa

Observed band size: 54 kDa

false

Exposure time: 3min

Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)
  • Dot

Unknown

Dot Blot - Anti-Vimentin (phospho S39) antibody [EP1069Y] (AB52942)

Dot blot analysis of Vimentin (pS39) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S39) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-Vimentin (phospho S39) antibody [EP1069Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1069Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/200000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 99% Tissue culture supernatant, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.
See full target information VIM phospho S39
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Poultry science 103:104146 PubMed39128391

2024

Cellular vimentin interacts with VP70 protein of goose astrovirus genotype 2 and acts as a structural organizer to facilitate viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Xiang,Linlin Li,Yunzhen Huang,Junqin Zhang,Jiawen Dong,Qi Zhai,Minhua Sun,Ming Liao

Acta pharmaceutica Sinica. B 14:2520-2536 PubMed38828143

2024

Interferon-induced MXB protein restricts vimentin-dependent viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Dongrong Yi,Ni An,Quanjie Li,Qian Liu,Huihan Shao,Rui Zhou,Jing Wang,Yongxin Zhang,Ling Ma,Fei Guo,Xiaoyu Li,Zhenlong Liu,Shan Cen

Frontiers in bioengineering and biotechnology 10:735465 PubMed35547156

2022

Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Guo,Yajie Chang,Jingjie Li,Chuanchuan Zhou,Rui Huang,Xing Yang,Guihua Liu,Xiaoyan Liang

JID innovations : skin science from molecules to population health 2:100076 PubMed35146482

2022

A Series of BRAF- and NRAS-Driven Murine Melanoma Cell Lines with Inducible Gene Modulation Capabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Ilah Bok,Ariana Angarita,Stephen M Douglass,Ashani T Weeraratna,Florian A Karreth

Science signaling 14:eabc8579 PubMed34609894

2021

Systems approach reveals distinct and shared signaling networks of the four PGE receptors in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anna M Lone,Piero Giansanti,Marthe Jøntvedt Jørgensen,Enio Gjerga,Aurelien Dugourd,Arjen Scholten,Julio Saez-Rodriguez,Albert J R Heck,Kjetil Taskén

Aging 13:957-972 PubMed33290258

2020

Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Yuyu Xie,Zhangbo Cui,Tian Xia,Lu Wan,Haifeng Zhou,Peng Zhang,Yijie Zhang,Fei Guan,Wenqi Liu,Chunwei Shi

Molecular biology of the cell 29:1555-1570 PubMed29718762

2018

In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

J L Walker,B M Bleaken,A R Romisher,A A Alnwibit,A S Menko

Oncology letters 12:4051-4053 PubMed27895770

2016

Classical intracranial chondrosarcoma: A case report.

Applications

IHC

Species

Human

Jingyang Chi,Mingchao Zhang,Jianmin Kang

Nature communications 5:4428 PubMed25079702

2014

Filamin acts as a key regulator in epithelial defence against transformed cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mihoko Kajita,Kaoru Sugimura,Atsuko Ohoka,Jemima Burden,Hitomi Suganuma,Masaya Ikegawa,Takashi Shimada,Tetsuya Kitamura,Masanobu Shindoh,Susumu Ishikawa,Sayaka Yamamoto,Sayaka Saitoh,Yuta Yako,Ryosuke Takahashi,Takaharu Okajima,Junichi Kikuta,Yumiko Maijima,Masaru Ishii,Masazumi Tada,Yasuyuki Fujita

PloS one 8:e73900 PubMed24073199

2013

Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells.

Applications

ICC/IF

Species

Human

Cong Haolong,Ning Du,Tian Hongchao,Yang Yang,Zhang Wei,Zhang Hua,Zhang Wenliang,Song Lei,Tien Po
View all publications
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