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Rabbit Recombinant Monoclonal Vimentin phospho S56 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 10 publications.

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Images

Flow Cytometry (Intracellular) - Anti-Vimentin (phospho S56) antibody [EPR21084] (AB217673), expandable thumbnail
  • Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (AB217673), expandable thumbnail
  • Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] (AB217673), expandable thumbnail
  • Western blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (AB217673), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] (AB217673), expandable thumbnail

Publications

  • Cancer cell international 22:2212022
    VEGF-B targeting by aryl hydrocarbon receptor mediates the migration and invasion of choriocarcinoma stem-like cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Qianxia Tan,Jingting Cai,Jingping Peng,Cui Hu,ChenChun Wu,Huining Liu
    PubMed 35773697
  • Biochemistry and biophysics reports 31:1013012022
    LINC01140 regulates osteosarcoma proliferation and invasion by targeting the miR-139-5p/HOXA9 axis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Shufang Zhang,Rongchun Chen
    PubMed 35800618

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

      Alternative names

      Vimentin, VIM

      Recommended products

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      Rabbit Recombinant Monoclonal Vimentin phospho S56 antibody. Suitable for IP, Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 10 publications.

      Alternative names

      Vimentin, VIM

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR21084

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Flow Cytometry (Intracellular) - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

        ab217673 staining Vimentin (phospho S56) in HeLa (human cervix adenocarcinoma epithelial cell) cells by intracellular flow cytometry. Cells were fixed using 80% Methanol and permeabilized with 0.1% Tween-20. The sample was incubated with primary antibody at 1/500 dilution. An Alexa Fluor®488 Goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as an isotype control (left). Cells were pre-treated with 20 μg/ml RNase A for 30 minutes to eliminate the non-specific binding between RNA and PI (Propidium iodide). Vimentiin (phospho S56) is highly expressed in mitotic cells. (PMID: 16260496)

      • Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673), expandable thumbnail

        Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

        Dot blot analysis of Vimentin (phospho S56) labeled with ab217673 at 1/1000 dilution.

        Lane 1: Vimentin (phospho S56) peptide.
        Lane 2: Vimentin non-phospho peptide.

        Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.

        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure time: 1 minute.

        The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

      • Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673), expandable thumbnail

        Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

        Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (Nocodazole, Microtubule inhibitor ab120630) for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

        Lane 1: HeLa treated with 100 ng/ml nocodazole (Nocodazole, Microtubule inhibitor ab120630) for 18 hours, whole cell lysate 10 μg (Input).
        Lane 2: ab217673 IP in HeLa treated with 100 ng/ml nocodazole (Nocodazole, Microtubule inhibitor ab120630) for 18 hours, whole cell lysate.
        Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole (Nocodazole, Microtubule inhibitor ab120630) for 18 hours, whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        All lanes: Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

        Developed using the ECL technique.

        Predicted band size: 53 kDa

        Observed band size: 57 kDa

        Exposure time: 1s

      • Western blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673), expandable thumbnail

        Western blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673) at 1/1000 dilution

        Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

        Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (Nocodazole, Microtubule inhibitor ab120630) for 18 hours, whole cell lysate at 10 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Developed using the ECL technique.

        Predicted band size: 53 kDa

        Observed band size: 57 kDa

        Exposure time: 23s

      • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin (phospho S56) with ab217673 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing strong positive staining in HeLa cells in M phase.

        The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

        Secondary antibody only control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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