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AB326643

Anti-Vimentin (phospho S72) antibody [SR1947]

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Rabbit Recombinant Monoclonal Vimentin phospho S72 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Vimentin, VIM

1 Images
Western blot - Anti-Vimentin (phospho S72) antibody [SR1947] (AB326643)
  • WB

Supplier Data

Western blot - Anti-Vimentin (phospho S72) antibody [SR1947] (AB326643)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : HeLa treated with 100nM Calyculin A for 30min whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Lane 3 : 3T3 treated with 100nM Calyculin A for 30min whole cell lysates
Lane 4 : C6 whole cell lysates
Lane 5 : C6 treated with 100nM Calyculin A for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa

Exposure time : 6 seconds

All lanes:

Western blot - Anti-Vimentin (phospho S72) antibody [SR1947] (ab326643) at 1/1000 dilution

Lane 1:

HeLa treated with 100nM Calyculin A for 30min whole cell lysates at 20 µg

Lane 2:

3T3 whole cell lysates at 20 µg

Lane 3:

3T3 treated with 100nM Calyculin A for 30min whole cell lysates at 20 µg

Lane 4:

C6 whole cell lysates at 20 µg

Lane 5:

C6 treated with 100nM Calyculin A for 30min whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1947

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
See full target information VIM pS72
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com