AB52944

Anti-Vimentin (phospho S73) antibody [EP1070Y]

RabMAb
Recombinant
Lab Essentials
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(7 Publications)

Rabbit Recombinant Monoclonal Vimentin phospho S73 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 7 publications.

View Alternative Names

Vimentin

6 Images

Unknown

Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

ab52944 (purified) at 1 : 20 dilution (2μg) immunoprecipitating Vimentin in HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate 10ug
Lane 2 (+) : ab52944 & HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52944 in HeLa starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate
For western blotting, VeriBlot for IP Detection Reageant (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)

Predicted band size: 53 kDa

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Unknown

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

All lanes:

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/1000000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Calyculin A at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 54 kDa

false

Unknown

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Blocking and diluting buffer : 5% NFDM/TBST

Lanes 1 - 2:

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution

Lanes 1 - 2:

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/vimentin-phospho-s73-antibody-ep1070y-bsa-and-azide-free-ab232451'>ab232451</a>) at 1/10000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate, untreated at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) starved for 24 hours, then treated with 100ng Calyculin A for 60 minutes, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

false

Unknown

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution

Lane 1:

Untreated with HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) starved for 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

false

Unknown

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution

Lane 1:

Untreated with NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml Calyculin A for 30 minutes whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

false

Unknown

Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Primary antibody dilution : 1/1000
Secondary antibody : goat anti-rabbit IgG, (H+L), peroxidase conjugated (1/2500 dilution)
Blocking & dilutiing buffer : 5% NFDM/TBST

Lane 1 sample : Vimentin (pS72) phospho peptide
Lane 2 sample : Vimentin non-phospho peptide

Exposure time : 3 minutes

Carrier free

Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1070Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Dot, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "For unpurified use at 1:40. ", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

See full target information VIM phospho S73

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012483 PubMed39226326

2024

Phosphorylated vimentin-triggered fibronectin matrix disaggregation enhances the dissemination of Treponema pallidum subsp. pallidum across the microvascular endothelial barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Luo,Litian Zhang,Xiaoyuan Xie,Liyan Yuan,Yanqiang Shi,Yinbo Jiang,Wujian Ke,Bin Yang

Acta pharmaceutica Sinica. B 14:2520-2536 PubMed38828143

2024

Interferon-induced MXB protein restricts vimentin-dependent viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Dongrong Yi,Ni An,Quanjie Li,Qian Liu,Huihan Shao,Rui Zhou,Jing Wang,Yongxin Zhang,Ling Ma,Fei Guo,Xiaoyu Li,Zhenlong Liu,Shan Cen

International journal of rheumatic diseases 27:e14990 PubMed38078507

2023

Phosphorylated vimentin at Ser72 is associated with epithelial-mesenchymal transition in lupus nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Daji Wang,Fan Zhang,Jing He,Chuyi Li,Xingjiao Liu,Meng Zhang,Haihui Huang,Zuying Xiong,Hongxia Duan,Xiaoyan Huang,Meiying Wang

Aging 13:957-972 PubMed33290258

2020

Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Yuyu Xie,Zhangbo Cui,Tian Xia,Lu Wan,Haifeng Zhou,Peng Zhang,Yijie Zhang,Fei Guan,Wenqi Liu,Chunwei Shi

Cancer immunology, immunotherapy : CII 69:989-999 PubMed32086539

2020

Phosphorylated vimentin as an immunotherapeutic target against metastatic colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mizuho Ohara,Kenzo Ohara,Takumi Kumai,Takayuki Ohkuri,Toshihiro Nagato,Yui Hirata-Nozaki,Akemi Kosaka,Marino Nagata,Ryusuke Hayashi,Shohei Harabuchi,Yuki Yajima,Kensuke Oikawa,Yasuaki Harabuchi,Yasuo Sumi,Hiroyuki Furukawa,Hiroya Kobayashi

Methods in enzymology 568:85-111 PubMed26795468

2016

Phospho-Specific Antibody Probes of Intermediate Filament Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Hidemasa Goto,Hiroki Tanaka,Kousuke Kasahara,Masaki Inagaki

Journal of biotechnology 145:111-9 PubMed19903502

2009

Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Anssi J Mähönen,Kaisa-Emilia Makkonen,Johanna P Laakkonen,Teemu O Ihalainen,Sari P Kukkonen,Minna U Kaikkonen,Maija Vihinen-Ranta,Seppo Ylä-Herttuala,Kari J Airenne

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vimentin (phospho S73) antibody [EP1070Y]

Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise