Rabbit Monoclonal Vimentin phospho S73 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 4 publications.
Images
![Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--immunoprecipitation-img9138.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)")
![Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--dot-blot-img11401.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)")
![Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--western-blot-img41626.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)")
![Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--western-blot-img15205.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (AB52944)")
Publications
Filter by
- Aging 13:957-9722020Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Liu et. al.PubMed 33290258
- Cancer immunology, immunotherapy : CII 69:989-9992020Phosphorylated vimentin as an immunotherapeutic target against metastatic colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMizuho Ohara,Kenzo Ohara,Takumi Kumai,Takayuki Ohkuri,Toshihiro Nagato,Yui Hirata-Nozaki,Akemi Kosaka,Marino Nagata,Ryusuke Hayashi,Shohei Harabuchi,Yuki Yajima,Kensuke Oikawa,Yasuaki Harabuchi,Yasuo Sumi,Hiroyuki Furukawa,Hiroya KobayashiPubMed 32086539
- Methods in enzymology 568:85-1112016Phospho-Specific Antibody Probes of Intermediate Filament Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHidemasa Goto,Hiroki Tanaka,Kousuke Kasahara,Masaki InagakiPubMed 26795468
- Journal of biotechnology 145:111-92009Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnssi J Mähönen,Kaisa-Emilia Makkonen,Johanna P Laakkonen,Teemu O Ihalainen,Sari P Kukkonen,Minna U Kaikkonen,Maija Vihinen-Ranta,Seppo Ylä-Herttuala,Kari J AirennePubMed 19903502
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Not recommended | Not recommended |
| Mouse | Expected | Expected | Tested | Not recommended | Not recommended |
| Rat | Expected | Expected | Tested | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes For unpurified use at 1:40. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 | Notes - |
Species Rat | Dilution info 1/10000 | Notes - |
Species Human | Dilution info 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Vimentin
Recommended products
Rabbit Monoclonal Vimentin phospho S73 antibody. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1070Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--immunoprecipitation-img9138.jpg "Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)")
Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
ab52944 (purified) at 1:20 dilution (2μg) immunoprecipitating Vimentin in HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate 10ug
Lane 2 (+): ab52944 & HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52944 in HeLa starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate
For western blotting, VeriBlot for IP Detection Reageant (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
Predicted band size: 53 kDa
![Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--dot-blot-img11401.jpg "Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)")
Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated (1/2500 dilution)
Blocking & dilutiing buffer: 5% NFDM/TBSTLane 1 sample: Vimentin (pS72) phospho peptide
Lane 2 sample: Vimentin non-phospho peptideExposure time: 3 minutes
![Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--western-blot-img105960.gif "Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)")
Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
All lanes: Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/1000000 dilution
Lane 1: HeLa cell lysate untreated at 10 µg
Lane 2: HeLa cell lysate treated with Calyculin A at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa
![Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--western-blot-img41626.jpg "Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)")
Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution
Lane 1: Untreated with HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved for 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
![Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--western-blot-img15205.jpg "Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)")
Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes: Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution
Lane 1: Untreated with NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml Calyculin A for 30 minutes whole cell lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
![Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--western-blot-img415599.jpg "Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)")
Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)
Blocking and diluting buffer : 5% NFDM/TBST
Lanes 1 - 2: Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944) at 1/10000 dilution
Lanes 1 - 2: Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free ab232451) at 1/10000 dilution
Lane 1: C6 (Rat glial tumor cell line) whole cell lysate, untreated at 15 µg
Lane 2: C6 (Rat glial tumor glial cell) starved for 24 hours, then treated with 100ng Calyculin A for 60 minutes, whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
![Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s73-antibody-ep1070y-ab52944--western-blot-img415599.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (ab52944)")
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