AB232451

Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Vimentin phospho S73 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

Vimentin

3 Images

Unknown

Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (AB232451)

ab52944 (purified) at 1 : 20 dilution (2μg) immunoprecipitating Vimentin in HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate 10ug
Lane 2 (+) : ab52944 & HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52944 in HeLa starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

All lanes:

Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] (<a href='/en-us/products/primary-antibodies/vimentin-phospho-s73-antibody-ep1070y-ab52944'>ab52944</a>)

Predicted band size: 53 kDa

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Unknown

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (AB232451)

Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

Lanes 1 - 2:

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] (<a href='/en-us/products/primary-antibodies/vimentin-phospho-s73-antibody-ep1070y-ab52944'>ab52944</a>) at 1/10000 dilution

Lanes 1 - 2:

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (ab232451) at 1/10000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate, untreated at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) starved for 24 hours, then treated with 100ng Calyculin A for 60 minutes, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

false

Unknown

Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (AB232451)

Primary antibody dilution : 1/1000
Secondary antibody : goat anti-rabbit IgG, (H+L), peroxidase conjugated (1/2500 dilution)
Blocking & dilutiing buffer : 5% NFDM/TBST

Lane 1 sample : Vimentin (pS72) phospho peptide
Lane 2 sample : Vimentin non-phospho peptide

Exposure time : 3 minutes

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52944).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1070Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "For unpurified use at 1:40.", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab232451 is the carrier-free version of ab52944.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vimentin, a class-III intermediate filament, is present in various non-epithelial cells, particularly mesenchymal cells, and connects either laterally or terminally to the nucleus, endoplasmic reticulum, and mitochondria. It is involved with LARP6 in stabilizing type I collagen mRNAs for CO1A1 and CO1A2. This supplementary information is collated from multiple sources and compiled automatically.

See full target information VIM phospho S73

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of medicinal chemistry 67:19282-19303 PubMed39450890

2024

Discovery and Characterization of BAY-184: A New Potent and Selective Acylsulfonamide-Benzofuran -Active KAT6AB Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Antonius Ter Laak,Roman C Hillig,Steven J Ferrara,Daniel Korr,Naomi Barak,Philip Lienau,Simon Herbert,Amaury Ernesto Fernández-Montalván,Roland Neuhaus,Mátyás Gorjánácz,Vera Puetter,Volker Badock,Wilhelm Bone,Craig Strathdee,Franziska Siegel,Christoph Schatz,Katrin Nowak-Reppel,Olaf Doehr,Stefan Gradl,Ingo V Hartung,Matthew Meyerson,Léa Bouché

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free

Immunoprecipitation - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (AB232451)

Western blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (AB232451)

Dot Blot - Anti-Vimentin (phospho S73) antibody [EP1070Y] - BSA and Azide free (AB232451)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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