AB52943

Anti-Vimentin (phospho S82) antibody [EP1071Y]

RabMAb
Lab Essentials
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(5 Publications)

Rabbit Monoclonal Vimentin phospho S82 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 5 publications.

View Alternative Names

Vimentin

2 Images

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Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (AB52943)

All lanes:

Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution

Lane 1:

HeLa cell lysate AP treated at 10 µg

Lane 2:

HeLa cell lysate (AP untreated) + Calyculin A at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 54 kDa

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Unknown

Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (AB52943)

Primary antibody dilution : 1/1000

Secondary antibody : goat anti-rabbit IgG, (H+L), peroxidase conjugated

Secondary antibody dilution : 1/2500

Blocking and dilution buffer : 5% NFDM/TBST

Lane 1 sample : Vimentin (pS83) phospho peptide

Lane 2 sample : Vimentin non-phospho peptide

Exposure time : 3 minutes

Carrier free

Anti-Vimentin (phospho S82) antibody [EP1071Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1071Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 99% Tissue culture supernatant, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

See full target information VIM phospho S82

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 14:2520-2536 PubMed38828143

2024

Interferon-induced MXB protein restricts vimentin-dependent viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Dongrong Yi,Ni An,Quanjie Li,Qian Liu,Huihan Shao,Rui Zhou,Jing Wang,Yongxin Zhang,Ling Ma,Fei Guo,Xiaoyu Li,Zhenlong Liu,Shan Cen

Aging 13:957-972 PubMed33290258

2020

Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Yuyu Xie,Zhangbo Cui,Tian Xia,Lu Wan,Haifeng Zhou,Peng Zhang,Yijie Zhang,Fei Guan,Wenqi Liu,Chunwei Shi

Methods in enzymology 568:85-111 PubMed26795468

2016

Phospho-Specific Antibody Probes of Intermediate Filament Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Hidemasa Goto,Hiroki Tanaka,Kousuke Kasahara,Masaki Inagaki

PloS one 8:e73900 PubMed24073199

2013

Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells.

Applications

ICC/IF, WB

Species

Human, Human

Cong Haolong,Ning Du,Tian Hongchao,Yang Yang,Zhang Wei,Zhang Hua,Zhang Wenliang,Song Lei,Tien Po

Journal of biotechnology 145:111-9 PubMed19903502

2009

Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Anssi J Mähönen,Kaisa-Emilia Makkonen,Johanna P Laakkonen,Teemu O Ihalainen,Sari P Kukkonen,Minna U Kaikkonen,Maija Vihinen-Ranta,Seppo Ylä-Herttuala,Kari J Airenne

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vimentin (phospho S82) antibody [EP1071Y]

Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (AB52943)

Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (AB52943)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

Product promise