Rabbit Monoclonal Vimentin phospho S82 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 4 publications.
Images
![Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (AB52943), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s82-antibody-ep1071y-ab52943--dot-blot-img6556.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (AB52943)")
Publications
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- Aging 13:957-9722020Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Liu et. al.PubMed 33290258
- Methods in enzymology 568:85-1112016Phospho-Specific Antibody Probes of Intermediate Filament Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHidemasa Goto,Hiroki Tanaka,Kousuke Kasahara,Masaki InagakiPubMed 26795468
- PloS one 8:e739002013Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells.
- Journal of biotechnology 145:111-92009Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnssi J Mähönen,Kaisa-Emilia Makkonen,Johanna P Laakkonen,Teemu O Ihalainen,Sari P Kukkonen,Minna U Kaikkonen,Maija Vihinen-Ranta,Seppo Ylä-Herttuala,Kari J AirennePubMed 19903502
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Predicted | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
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Target data
Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Alternative names
Vimentin
Recommended products
Rabbit Monoclonal Vimentin phospho S82 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1071Y
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s82-antibody-ep1071y-ab52943--dot-blot-img6556.jpg "Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)")
Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)
Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking and dilution buffer: 5% NFDM/TBST
Lane 1 sample: Vimentin (pS83) phospho peptide
Lane 2 sample: Vimentin non-phospho peptide
Exposure time: 3 minutes
![Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943), expandable thumbnail](https://content.abcam.com/products/images/vimentin-phospho-s82-antibody-ep1071y-ab52943--western-blot-img29545.gif "Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)")
Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)
All lanes: Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution
Lane 1: HeLa cell lysate AP treated at 10 µg
Lane 2: HeLa cell lysate (AP untreated) + Calyculin A at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa
Downloads
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