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Rabbit Recombinant Monoclonal Vinculin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-Vinculin antibody [EPR19579] (AB207440), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [EPR19579] (AB207440), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [EPR19579] (AB207440), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [EPR19579] (AB207440), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [EPR19579] (AB207440), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Alternative names

Vinculin, Metavinculin, MV, VCL

Recommended products

Rabbit Recombinant Monoclonal Vinculin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19579
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Vinculin antibody [EPR19579] (ab207440), expandable thumbnail

    Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

    Lanes 1-4: Merged signal (red and green). Green - ab207440 observed at 124 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab207440 Anti-Vinculin antibody [EPR19579] was shown to specifically react with Vinculin in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human VCL (Vinculin) knockout HeLa cell line ab265580 (knockout cell lysate Human VCL (Vinculin) knockout HeLa cell lysate ab257795) was used. Wild-type and Vinculin knockout samples were subjected to SDS-PAGE. ab207440 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: Vinculin knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human VCL (Vinculin) knockout HeLa cell line (Human VCL (Vinculin) knockout HeLa cell line ab265580)

    Lane 3: HEK-293 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 124 kDa

    Observed band size: 124 kDa

  • Western blot - Anti-Vinculin antibody [EPR19579] (ab207440), expandable thumbnail

    Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

    Lanes 1 - 4: Merged signal (red and green). Green - ab207440 observed at 124 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab207440 was shown to react with VCL in A431 wild-type cells in Western blot. Loss of signal was observed when VCL knockout sample was used. A431 wild-type and VCL knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab207440 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

    Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: VCL knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human VCL (Vinculin) knockout A-431 cell line (Human VCL (Vinculin) knockout A-431 cell line ab261898)

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 124 kDa

    Observed band size: 124 kDa

  • Western blot - Anti-Vinculin antibody [EPR19579] (ab207440), expandable thumbnail

    Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 3 minutes; Lane 5/6: 5 seconds.

    All lanes: Western blot - Anti-Vinculin antibody [EPR19579] (ab207440) at 1/2000 dilution

    Lane 1: K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 10 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 3: HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

    Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 5: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 6: Human testis lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 124 kDa

    Observed band size: 124 kDa

  • Western blot - Anti-Vinculin antibody [EPR19579] (ab207440), expandable thumbnail

    Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

    Lane 1: Human fetal heart lysate at 10 µg

    Lane 2: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 124 kDa

    Observed band size: 124 kDa

    Exposure time: 30s

  • Western blot - Anti-Vinculin antibody [EPR19579] (ab207440), expandable thumbnail

    Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-3: 30 seconds; Lane 4-8: 10 seconds.

    All lanes: Western blot - Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

    Lane 1: Mouse heart lysate at 10 µg

    Lane 2: Mouse kidney lysate at 10 µg

    Lane 3: Mouse spleen lysate at 10 µg

    Lane 4: Rat kidney lysate at 10 µg

    Lane 5: Rat spleen lysate at 10 µg

    Lane 6: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 7: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 8: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 124 kDa

    Observed band size: 124 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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