Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--western-blot-img52243.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109837.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121452.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116090.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130035.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (AB240395)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Alternative names
Vinculin, Metavinculin, MV, VCL
Recommended products
Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20407
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240395 is the carrier-free version of Anti-Vinculin antibody [EPR20407] ab219649.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--western-blot-img52243.jpg "Western blot - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
Western blot - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Vinculin antibody [EPR20407] ab219649).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Vinculin antibody [EPR20407] ab219649 observed at 124 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-Vinculin antibody [EPR20407] ab219649 was shown to react with VCL in A431 wild-type cells in Western blot. Loss of signal was observed when VCL knockout sample was used. A431 wild-type and VCL knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with Anti-Vinculin antibody [EPR20407] ab219649 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Vinculin antibody [EPR20407] (Anti-Vinculin antibody [EPR20407] ab219649) at 1/1000 dilution
Lane 1: Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 2: VCL knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Western blot - Human VCL (Vinculin) knockout A-431 cell line (Human VCL (Vinculin) knockout A-431 cell line ab261898)
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 124 kDa
Observed band size: 124 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109837.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Vinculin with Anti-Vinculin antibody [EPR20407] ab219649 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on myoepithelium of human breast is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR20407] ab219649).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121452.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Vinculin with Anti-Vinculin antibody [EPR20407] ab219649 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous and cytoplasmic staining on smooth muscle cells of human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR20407] ab219649).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116090.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling Vinculin with Anti-Vinculin antibody [EPR20407] ab219649 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous and cytoplasmic staining on smooth muscle cells of human gastric cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR20407] ab219649).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130035.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Vinculin with Anti-Vinculin antibody [EPR20407] ab219649 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous and cytoplasmic staining on smooth muscle cells of human prostate cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR20407] ab219649).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23648.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Vinculin with Anti-Vinculin antibody [EPR20407] ab219649 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous and cytoplasmic staining on smooth muscle cells of mouse colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR20407] ab219649).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117402.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Vinculin with Anti-Vinculin antibody [EPR20407] ab219649 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous and cytoplasmic staining on smooth muscle cells of rat stomach is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR20407] ab219649).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23648.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr20407-bsa-and-azide-free-ab240395--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117402.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [EPR20407] - BSA and Azide free (ab240395)")
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