Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Images
![Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--immunoprecipitation-img7981.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171)")
![Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--immunocytochemistry-immunofluorescence-img118915.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171)")
![Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--flow-cytometry-intracellular-img50441.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171)")
![Western blot - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--western-blot-img168330.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171)")
![Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--immunocytochemistry-immunofluorescence-img41732.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (AB217171)")
Publications
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- The EMBO journal 40:e1085882021A functional assay for serum detection of antibodies against SARS-CoV-2 nucleoprotein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Albecka,Dean Clift,Marina Vaysburd,Tyler Rhinesmith,Sarah L Caddy,David M Favara,Helen E Baxendale,Leo C JamesPubMed 34323299
- PloS one 11:e01633142016Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice.
- American journal of physiology. Renal physiology 311:F362-722016Delivery of interleukin-10 via injectable hydrogels improves renal outcomes and reduces systemic inflammation following ischemic acute kidney injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanielle E Soranno et. al.PubMed 26962109
- PloS one 10:e01449242015Global miRNA Expression Profiling Identifies miR-1290 as Novel Potential oncomiR in Laryngeal Carcinoma.Applications:WBReactive species:Unspecified reactive speciesJoanna Janiszewska et. al.PubMed 26694163
- Biophysical journal 109:57-652015The cytoskeleton regulates cell attachment strength.Applications:WBReactive species:Unspecified reactive speciesAlexander Fuhrmann,Adam J EnglerPubMed 26153702
- Oncotarget 5:6701-152014DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-Rho GTPase signaling.Applications:IFReactive species:HumanYing Fu,Ming-Xuan Feng,Jian Yu,Ming-Ze Ma,Xiao-Jin Liu,Jun Li,Xiao-Mei Yang,Ya-Hui Wang,Yan-Li Zhang,Jun-Ping Ao,Feng Xue,Wenxin Qin,Jianren Gu,Qiang Xia,Zhi-Gang ZhangPubMed 25149533
- Stem cells (Dayton, Ohio) 31:2467-772013In situ mechanotransduction via vinculin regulates stem cell differentiation.Applications:ICC/IFReactive species:HumanAndrew W Holle,Xinyi Tang,Deepthi Vijayraghavan,Ludovic G Vincent,Alexander Fuhrmann,Yu Suk Choi,Juan C del Álamo,Adam J EnglerPubMed 23897765
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Expected | Tested | Not recommended | Expected | Expected |
| Rat | Expected | Tested | Not recommended | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
3 products for Alternative Version
Target data
Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Alternative names
Vinculin, Metavinculin, MV, VCL
Recommended products
Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8185
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab217171 is the carrier-free version of Anti-Vinculin antibody [EPR8185] - Loading Control ab129002.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--immunoprecipitation-img7981.jpg "Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)")
Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 (purified) at 1/20 immunoprecipitating vinculin in HeLa cells. Lane 1: HeLa whole cell lysate (10 µg). Lane 2: HeLa whole cell lysate (10 µg). Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 in HeLa whole cell lysate. For western blotting, a HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002).
All lanes: Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - Loading Control (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002)
Predicted band size: 124 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--immunocytochemistry-immunofluorescence-img118915.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)")
Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
Vinculin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Vinculin antibody
Immunofluorescent staining of HEK293 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary at a dilution of 1/1000 and the cells were counter stained with DAPI. The negative controls are shown in the bottom middle and right hand panels. For negative control 1, the primary was used and then goat anti-mouse IgG was used at a dilution of 1/500. For negative control 2, a mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) were used.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002).
![Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--flow-cytometry-intracellular-img50441.jpg "Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)")
Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
Vinculin Flow Cytometry (Intracellular) staining using rabbit Anti-Vinculin antibody
Intracellular Flow Cytometry analysis of 293 (human embryonic kidney epithelial) cells labeling Vinculin (red) with Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002).
![Western blot - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--western-blot-img168330.jpg "Western blot - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)")
Western blot - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
Vinculin Western blot staining using rabbit Anti-Vinculin antibody
This data was developed using Anti-Vinculin antibody [EPR8185] - Loading Control ab129002, the same antibody clone in a different buffer formulation. Different batches of Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 were tested on HepG2 (Human hepatocellular carcinoma epithelial cell) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 124 kDa.
All lanes: Western blot - Anti-Vinculin antibody [EPR8185] - Loading Control (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002)
Predicted band size: 124 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--immunocytochemistry-immunofluorescence-img41732.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)")
Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
Vinculin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Vinculin antibody
Immunofluorescent staining of vinculin in HUVEC cells with unpurified Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002).
![Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--flow-cytometry-intracellular-img421256.jpg "Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)")
Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
Vinculin Flow Cytometry (Intracellular) staining using rabbit Anti-Vinculin antibody
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002).
Flow cytometry overlay histogram showing wild-type A-431 (green line) and VCL knockout A-431 stained with Anti-Vinculin antibody [EPR8185] - Loading Control ab129002 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) (1x 106 in 100μl at 0.00032 μg/ml (1/6249999)) for 30min at 22°C.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C
Isotype control antibody Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control was used at the same concentration and conditions as the primary antibody (wild-type A-431 - black line, VCL knockout A-431 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
![Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-epr8185-bsa-and-azide-free-ab217171--flow-cytometry-intracellular-img421256.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)")
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