Anti-Vinculin antibody [HL1873] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865)

Paraffin-embedded mouse stomach tissue stained for Vinculin protein at cytoplasm with ab313865 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865)

All lanes:

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Lane 5:

NCI-H1299 (Human lung carcinoma cell line) whole cell extract at 30 µg

Lane 6:

HCT116 (Human colorectal carcinoma cell line) whole cell extract at 30 µg

Lane 7:

MCF7 (Human breast adenocarcinoma cell line) whole cell extract at 30 µg

Lane 8:

NTERA-2 cl.D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg

Lane 9:

PC-3 (Human prostate adenocarcinoma cell line) whole cell extract at 30 µg

Lane 10:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false

• WB

Supplier Data

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 123 kDa

false

• WB

Supplier Data

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865)

All lanes:

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/1000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false

• WB

Supplier Data

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865)

All lanes:

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false

• WB

Supplier Data

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865)

All lanes:

Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution

Lane 1:

MDCK whole cell extract at 30 µg

Lane 2:

PG-4 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 124 kDa

false