Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Dog, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL.
pH: 7.4
Constituents: 100% PBS
WB | IHC-P | |
---|---|---|
Human | Tested | Expected |
Mouse | Tested | Tested |
Rat | Tested | Expected |
Cat | Tested | Expected |
Dog | Tested | Expected |
Zebrafish | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Zebrafish | Dilution info 1/500 - 1/3000 | Notes - |
Species Dog | Dilution info 1/500 - 1/3000 | Notes - |
Species Cat | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Zebrafish, Dog, Cat | Dilution info Use at an assay dependent concentration. | Notes - |
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Vinc, Vcl
Vinculin, Metavinculin, MV, VCL
Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Dog, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL.
pH: 7.4
Constituents: 100% PBS
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Paraffin-embedded mouse stomach tissue stained for Vinculin protein at cytoplasm with ab313865 at a 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Samples were seperated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution
Lane 1: HeLa whole cell extracts at 30 µg
Lane 2: HepG2 whole cell extracts at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 123 kDa
All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/1000 dilution
Lane 1: Neuro-2a (Mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 124 kDa
All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution
Lane 1: MDCK whole cell extract at 30 µg
Lane 2: PG-4 whole cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 124 kDa
All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/1000 dilution
All lanes: Whole zebrafish extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 124 kDa
All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Lane 5: NCI-H1299 (Human lung carcinoma cell line) whole cell extract at 30 µg
Lane 6: HCT116 (Human colorectal carcinoma cell line) whole cell extract at 30 µg
Lane 7: MCF7 (Human breast adenocarcinoma cell line) whole cell extract at 30 µg
Lane 8: NTERA-2 cl.D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg
Lane 9: PC-3 (Human prostate adenocarcinoma cell line) whole cell extract at 30 µg
Lane 10: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 124 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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