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Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Dog, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (AB313865), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human VCL. Database link P18206

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Expected
Mouse
Tested
Tested
Rat
Tested
Expected
Cat
Tested
Expected
Dog
Tested
Expected
Zebrafish
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/500 - 1/3000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Species
Zebrafish
Dilution info
1/500 - 1/3000
Notes

-

Species
Dog
Dilution info
1/500 - 1/3000
Notes

-

Species
Cat
Dilution info
1/500 - 1/3000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat, Zebrafish, Dog, Cat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Additional Targets

Vinc, Vcl

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Vinculin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Dog, Cat samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human VCL. Database link P18206
Carrier free
Yes
Clone number
HL1873
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865)

    Paraffin-embedded mouse stomach tissue stained for Vinculin protein at cytoplasm with ab313865 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865), expandable thumbnail

    Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865)

    Samples were seperated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

    All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution

    Lane 1: HeLa whole cell extracts at 30 µg

    Lane 2: HepG2 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 123 kDa

  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865), expandable thumbnail

    Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865)

    All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/1000 dilution

    Lane 1: Neuro-2a (Mouse neuroblastoma cell line) whole cell extract at 30 µg

    Lane 2: Rat2 (Rat fibroblast cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 124 kDa

  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865), expandable thumbnail

    Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865)

    All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution

    Lane 1: MDCK whole cell extract at 30 µg

    Lane 2: PG-4 whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 124 kDa

  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865), expandable thumbnail

    Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865)

    All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/1000 dilution

    All lanes: Whole zebrafish extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 124 kDa

  • Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865), expandable thumbnail

    Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865)

    All lanes: Western blot - Anti-Vinculin antibody [HL1873] - BSA and Azide free (ab313865) at 1/3000 dilution

    Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg

    Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

    Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

    Lane 5: NCI-H1299 (Human lung carcinoma cell line) whole cell extract at 30 µg

    Lane 6: HCT116 (Human colorectal carcinoma cell line) whole cell extract at 30 µg

    Lane 7: MCF7 (Human breast adenocarcinoma cell line) whole cell extract at 30 µg

    Lane 8: NTERA-2 cl.D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg

    Lane 9: PC-3 (Human prostate adenocarcinoma cell line) whole cell extract at 30 µg

    Lane 10: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 124 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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