AB155120

Anti-Vinculin antibody - Loading Control

Name: Anti-Vinculin antibody. Rabbit polyclonal (ab155120) | Abcam
Brand: Abcam Limited
SKU: AB155120
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(17 Publications)

Rabbit Polyclonal Vinculin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VCL aa 50-350.

View Alternative Names

Vinculin, Metavinculin, MV, VCL

8 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody - Loading Control (AB155120)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling Vinculin protein at focal adhesion using ab155120 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).
Scale bar = 10 µM

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody - Loading Control (AB155120)

Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue, labeling Vinculin with ab155120 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody - Loading Control (AB155120)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Vinculin protein at cytoplasm with ab155120 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Vinculin antibody - Loading Control (ab155120) at 1/1000 dilution

All lanes:

NT2D1 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Vinculin antibody - Loading Control (ab155120) at 1/10000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Vinculin antibody - Loading Control (ab155120) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Vinculin antibody - Loading Control (ab155120) at 1/1000 dilution

Lane 1:

Neuro2A whole cell extracts at 50 µg

Lane 2:

Rat2 whole cell extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 124 kDa

false

Supplier Data

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Vinculin antibody - Loading Control (ab155120) at 1/1000 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 124 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VCL aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P18206

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

See full target information VCL

style

left-column

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 51:858-875 PubMed40678259

2025

Lung cancer intravasation-on-a-chip: Visualization and machine learning-assisted automatic quantification.

Applications

Unspecified application

Species

Unspecified reactive species

Christy Wing Tung Wong,Joyce Zhi Xuen Lee,Anna Jaeschke,Sammi Sze Ying Ng,Kwok Keung Lit,Ho-Ying Wan,Caroline Kniebs,Dai Fei Elmer Ker,Rocky S Tuan,Anna Blocki

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 40:133-150 PubMed38794880

2024

Propionate and butyrate counteract renal damage and progression to chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana Corte-Iglesias,Maria Laura Saiz,Ana Cristina Andrade-Lopez,Nuria Salazar,Cristian Ruiz Bernet,Cristina Martin-Martin,Jesús Martinez Borra,Juan-Jose Lozano,Ana M Aransay,Carmen Diaz-Corte,Carlos Lopez-Larrea,Beatriz Suarez-Alvarez

International journal of molecular sciences 24: PubMed37686378

2023

Enhanced Angiogenesis in HUVECs Preconditioned with Media from Adipocytes Differentiated from Lipedema Adipose Stem Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Al-Ghadban,Samantha G Walczak,Spencer U Isern,Elizabeth C Martin,Karen L Herbst,Bruce A Bunnell

Frontiers in physiology 14:1218924 PubMed37637147

2023

The effect of cyclic stretch on aortic viscoelasticity and the putative role of smooth muscle focal adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Cédric H G Neutel,Callan D Wesley,Guido R Y De Meyer,Wim Martinet,Pieter-Jan Guns

Frontiers in genetics 14:1209138 PubMed37547463

2023

A self-repair history: compensatory effect of a variant on the c.2778+83C>G splicing mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Persico,Giorgia Fontana,Michela Faleschini,Melania Eva Zanchetta,Daniele Ammeti,Enrico Cappelli,Fabio Corsolini,Clara Mosa,Angela Guarina,Massimo Bogliolo,Jordi Surrallés,Carlo Dufour,Piero Farruggia,Anna Savoia,Roberta Bottega

Cells 12: PubMed37190104

2023

Inducible MLL-AF9 Expression Drives an AML Program during Human Pluripotent Stem Cell-Derived Hematopoietic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Branco M H Heuts,Saioa Arza-Apalategi,Sinne G Alkema,Esther Tijchon,Laura Jussen,Saskia M Bergevoet,Bert A van der Reijden,Joost H A Martens

Biomaterials research 27:32 PubMed37076899

2023

Stabilization and improved functionality of three-dimensional perfusable microvascular networks in microfluidic devices under macromolecular crowding.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Ying Wan,Jack Chun Hin Chen,Qinru Xiao,Christy Wingtung Wong,Boguang Yang,Benjamin Cao,Rocky S Tuan,Susan K Nilsson,Yi-Ping Ho,Michael Raghunath,Roger D Kamm,Anna Blocki

iScience 26:106158 PubMed36843839

2023

Telomeres cooperate in zygotic genome activation by affecting / transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorui Zhang,Changquan Zhang,Di Zhou,Tianlei Zhang,Xueqin Chen,Jinlin Ren,Caixia He,Fei Meng,Qinwei Zhou,Qiaohui Yang,Congling Dai,Ge Lin,Sicong Zeng,Lizhi Leng

Human gene therapy 34:459-470 PubMed36310439

2022

The Implication of Hinge 1 and Hinge 4 in Micro-Dystrophin Gene Therapy for Duchenne Muscular Dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Lakmini P Wasala,Thais B Watkins,Nalinda B Wasala,Matthew J Burke,Yongping Yue,Yi Lai,Gang Yao,Dongsheng Duan

Cancer chemotherapy and pharmacology 90:345-356 PubMed36050497

2022

Potentiation of temozolomide activity against glioblastoma cells by aromatase inhibitor letrozole.

Applications

Unspecified application

Species

Unspecified reactive species

Aniruddha S Karve,Janki M Desai,Nimita Dave,Trisha M Wise-Draper,Gary A Gudelsky,Timothy N Phoenix,Biplab DasGupta,Soma Sengupta,David R Plas,Pankaj B Desai

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vinculin antibody - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody - Loading Control (AB155120)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody - Loading Control (AB155120)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody - Loading Control (AB155120)

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Western blot - Anti-Vinculin antibody - Loading Control (AB155120)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (17)

Product promise