Anti-Vinculin antibody (ab91459) is a rabbit polyclonal antibody detecting Vinculin in Western Blot, IP, IHC-P. Suitable for Human, Mouse.
- Over 10 publications
- Trusted since 2010
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- Biology direct 18:652023Kruppel-like factor 13 acts as a tumor suppressor in thyroid carcinoma by downregulating IFIT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Liu,Yixuan Song,Yuqin He,Ziren Kong,Han Li,Yiming Zhu,Shaoyan LiuPubMed 37817224
- STAR protocols 4:1019502022Adeno-associated viral delivery of engineered tRNA-enzyme pairs into nonsense mutation mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhetao Zheng,Ningning Shi,Qing XiaPubMed 36527714
- The Journal of clinical investigation 131:2021LIN28B alters ribosomal dynamics to promote metastasis in MYCN-driven malignancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavlos Missios,Edroaldo Lummertz da Rocha,Daniel S Pearson,Julia Philipp,Maria M Aleman,Mehdi Pirouz,Dorian Farache,Joseph W Franses,Caroline Kubaczka,Kaloyan M Tsanov,Deepak K Jha,Brian Pepe-Mooney,John T Powers,Richard I Gregory,Amy Sy Lee,Daniel Dominguez,David T Ting,George Q DaleyPubMed 34779407
- Aging 13:23981-240162021Restoration of aged hematopoietic cells by their young counterparts through instructive microvesicles release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteven J Greco,Seda Ayer,Khadidiatou Guiro,Garima Sinha,Robert J Donnelly,Markos H El-Far,Lauren S Sherman,Yannick Kenfack,Sri Harika Pamarthi,Marina Gergues,Oleta A Sandiford,Michael J Schonning,Jean-Pierre Etchegaray,Pranela RameshwarPubMed 34762598
- Development (Cambridge, England) 148:2021Anterior expansion and posterior addition to the notochord mechanically coordinate zebrafish embryo axis elongation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusannah B P McLaren,Benjamin J SteventonPubMed 34086031
- eLife 10:2021A pentameric protein ring with novel architecture is required for herpesviral packaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAllison L Didychuk et. al.PubMed 33554858
- The Journal of experimental biology 223:2020insulin treatment reverses changes elicited by nutrients in cellular metabolic processes that regulate food intake in fish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyelén M Blanco et. al.PubMed 32179544
- Translational medicine of aging 4:22-312020Remodeling of the H3 nucleosomal landscape during mouse aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYilin Chen et. al.PubMed 32462102
- PloS one 11:e01503232016Paxillin and Focal Adhesion Kinase (FAK) Regulate Cardiac Contractility in the Zebrafish Heart.
- The Journal of biological chemistry 289:10650-92014Bacillus cereus Certhrax ADP-ribosylates vinculin to disrupt focal adhesion complexes and cell adhesion.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human VCL aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18206
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Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Medaka fish | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Platypus | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Tilapia | Predicted | Predicted | Predicted |
| Turkey | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-15 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 - 1/10000 | Notes - |
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus | Dilution info - | Notes - |
Associated Products
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Target data
Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Alternative names
Vinculin, Metavinculin, MV, VCL
Recommended products
Anti-Vinculin antibody (ab91459) is a rabbit polyclonal antibody detecting Vinculin in Western Blot, IP, IHC-P. Suitable for Human, Mouse.
- Over 10 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human VCL aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18206
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Vinculin antibody (ab91459) is a rabbit polyclonal antibody and is validated for use in IHC-P, IP, WB in human, mouse samples.
Anti-Vinculin antibody (ab91459) specifically detects Vinculin (UniProt ID: P18206; Molecular weight: 124kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Vinculin antibody (ab91459) has high sensitivity and specificity.
Anti-Vinculin antibody (ab91459) has been cited over 11 times in peer reviewed journals and is trusted by the scientific community.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-Vinculin antibody - Loading Control (ab91459)
Vinculin Western blot staining using rabbit Anti-Vinculin antibody
All lanes: Western blot - Anti-Vinculin antibody - Loading Control (ab91459) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 3min
Immunoprecipitation - Anti-Vinculin antibody - Loading Control (ab91459)
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10μg ab91459.
20% of the immunoprecipitate was loaded per lane, and probed with ab91459 at 1μg/ml (lane 1) or a control IgG (lane2).
Detection: chemoluminescence with an exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-Vinculin antibody - Loading Control (ab91459)
Predicted band size: 124 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody - Loading Control (ab91459)
Vinculin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Vinculin antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Vinculin with ab91459 at 1/1000 (1µg/ml). Detection: DAB.
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