AB91459

Anti-Vinculin antibody - Loading Control

Name: Vinculin antibody, rabbit polyclonal (ab91459) | Abcam
Brand: Abcam Limited
SKU: AB91459
Price: 420 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(32 Publications)

Anti-Vinculin antibody (ab91459) is a rabbit polyclonal antibody detecting Vinculin in Western Blot, IP, IHC-P. Suitable for Human, Mouse.

- Over 10 publications
- Trusted since 2010

View Alternative Names

Vinculin, Metavinculin, MV, VCL

3 Images

Unknown

Western blot - Anti-Vinculin antibody - Loading Control (AB91459)

All lanes:

Western blot - Anti-Vinculin antibody - Loading Control (ab91459) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 whole cell lysate at 50 µg

Predicted band size: 124 kDa

true

Exposure time: 3min

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody - Loading Control (AB91459)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Vinculin with ab91459 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-Vinculin antibody - Loading Control (AB91459)

SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10μg ab91459.
20% of the immunoprecipitate was loaded per lane, and probed with ab91459 at 1μg/ml (lane 1) or a control IgG (lane2).
Detection : chemoluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Vinculin antibody - Loading Control (ab91459)

Predicted band size: 124 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human VCL aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P18206

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-15 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Medaka fish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tilapia": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Product Specifications
Anti-Vinculin antibody (ab91459) is a rabbit polyclonal antibody and is validated for use in IHC-P, IP, WB in human, mouse samples.
Anti-Vinculin antibody (ab91459) specifically detects Vinculin (UniProt ID: P18206; Molecular weight: 124kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Vinculin antibody (ab91459) has high sensitivity and specificity.
Anti-Vinculin antibody (ab91459) has been cited over 11 times in peer reviewed journals and is trusted by the scientific community.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

See full target information VCL

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:47 PubMed40833324

2025

Insights Into the FOXE3 Transcriptional Network and Disease Mechanisms From the Investigation of a Regulatory Variant Driving Complex Microphthalmia.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Plaisancié,Clémentine Angée,Elisa Erjavec,Isabelle Raymond-Letron,Jean-Yves Douet,Mathilde Goetz,Catherine Vincent-Delorme,Ino D Karemaker,Marijke Baltissen,Michiel Vermeulen,Leonardo Valdivia,Fabienne Jabot-Hanin,Pierre David,Djihad Hadjadj,Yanad Abou Monsef,Faouzi Lyazrhi,Patrick Calvas,Jean-Michel Rozet,Nicolas Chassaing,Lucas Fares-Taie

Proceedings of the National Academy of Sciences of the United States of America 122:e2507186122 PubMed40768347

2025

RNA polymerase III transcription-associated polyadenylation promotes the accumulation of noncoding retrotransposons during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Azra Lari,Sahil B Shah,Xiaowen Mao,Priyanka Sanghrajka,John Karijolich,Liana F Lareau,Britt A Glaunsinger

Molecular psychiatry 30:4591-4604 PubMed40346285

2025

CLN5 deficiency impairs glucose uptake and uncovers PHGDH as a potential biomarker in Batten disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Marchese,Sara Bernardi,Rachele Vivarelli,Stefano Doccini,Lorenzo Santucci,Asahi Ogi,Rosario Licitra,Jingjing Zang,Rabah Soliymani,Serena Mero,Stephan Cf Neuhauss,Lea Ciarmoli,Giovanni Signore,Maciej M Lalowski,Filippo M Santorelli

Molecular neurodegeneration 20:4 PubMed39806441

2025

RNA-Targeting CRISPR/CasRx system relieves disease symptoms in Huntington's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqi Lin,Caijuan Li,Yizhi Chen,Jiale Gao,Jiawei Li,Chunhui Huang,Zhaoming Liu,Wei Wang,Xiao Zheng,Xichen Song,Jianhao Wu,Jiaxi Wu,Oscar Junhong Luo,Zhuchi Tu,Shihua Li,Xiao-Jiang Li,Liangxue Lai,Sen Yan

eLife 13: PubMed39625470

2024

TPR is required for cytoplasmic chromatin fragment formation during senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Bethany M Bartlett,Yatendra Kumar,Shelagh Boyle,Tamoghna Chowdhury,Andrea Quintanilla,Charlene Boumendil,Juan Carlos Acosta,Wendy A Bickmore

NPJ Parkinson's disease 10:192 PubMed39438499

2024

Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2.

Applications

Unspecified application

Species

Unspecified reactive species

Kathi Ging,Lukas Frick,Johannes Schlachetzki,Andrea Armani,Yanping Zhu,Pierre-André Gilormini,Ashutosh Dhingra,Desirée Böck,Ana Marques,Matthew Deen,Xi Chen,Tetiana Serdiuk,Chiara Trevisan,Stefano Sellitto,Claudio Pisano,Christopher K Glass,Peter Heutink,Jiang-An Yin,David J Vocadlo,Adriano Aguzzi

Cell death and differentiation 31:1611-1624 PubMed39406918

2024

AKR1B1-dependent fructose metabolism enhances malignancy of cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Zhao,Bing Han,Lu Wang,Jia Wu,Siliang Wang,Zhenxing Ren,Shouli Wang,Haining Yang,Michele Carbone,Changsheng Dong,Gerry Melino,Wen-Lian Chen,Wei Jia

Nature communications 15:8182 PubMed39294148

2024

β2 integrins impose a mechanical checkpoint on macrophage phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander H Settle,Benjamin Y Winer,Miguel M de Jesus,Lauren Seeman,Zhaoquan Wang,Eric Chan,Yevgeniy Romin,Zhuoning Li,Matthew M Miele,Ronald C Hendrickson,Daan Vorselen,Justin S A Perry,Morgan Huse

Cytoskeleton (Hoboken, N.J.) 82:444-455 PubMed38984538

2024

A truncation mutant of adenomatous polyposis coli impairs apical cell extrusion through elevated epithelial tissue tension.

Applications

Unspecified application

Species

Unspecified reactive species

Wan J Gan,Rabina Giri,Jakob Begun,Helen E Abud,Edna C Hardeman,Peter W Gunning,Alpha S Yap,Ivar Noordstra

iScience 27:109236 PubMed38487019

2024

Mechanisms of HIV-mediated blood-brain barrier compromise and leukocyte transmigration under the current antiretroviral era.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian Hernandez,Anna Maria Gorska,Eliseo Eugenin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vinculin antibody - Loading Control

Western blot - Anti-Vinculin antibody - Loading Control (AB91459)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody - Loading Control (AB91459)

Immunoprecipitation - Anti-Vinculin antibody - Loading Control (AB91459)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (32)

Product promise