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AB111004

Anti-Vinculin antibody [SP117] - C-terminal

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Vinculin antibody. C-terminal. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

Vinculin, Metavinculin, MV, VCL

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human heart tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Vinculin with ab111004 at 1/100 dilution (1.84 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle in human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab111004 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human breast adenocarcinoma tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human colon tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human breast tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human bladder tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - C-terminal (AB111004)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling Vinculin with ab111004 at 1/100 dilution (1.84 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle in rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab111004 for 10 mins at room temperature.

  • Carrier free

    Anti-Vinculin antibody [SP117] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP117

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by Protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
See full target information VCL

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:780727 PubMed34956208

2021

NFAT5 Controls the Integrity of Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid Muhammad,Delicia Xavier,Stefan Klein-Hessling,Muhammad Azeem,Tabea Rauschenberger,Krisna Murti,Andris Avots,Matthias Goebeler,Matthias Klein,Tobias Bopp,Malte Sielaff,Stefan Tenzer,Sigrid Möckel,José Aramburu,Cristina López-Rodríguez,Andreas Kerstan,Edgar Serfling
View all publications

Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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