Mouse Monoclonal Vinculin antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL aa 150-350.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (AB269680), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-vcl-2575-ab269680--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109338.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (AB269680)")
![Western blot - Anti-Vinculin antibody [VCL/2575] (AB269680), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-vcl-2575-ab269680--western-blot-img37028.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Vinculin antibody [VCL/2575] (AB269680)")
![Protein Array - Anti-Vinculin antibody [VCL/2575] (AB269680), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-vcl-2575-ab269680--protein-array-img141660.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Vinculin antibody [VCL/2575] (AB269680)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human VCL aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P18206
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
6 products for Alternative Product
Target data
Function
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Alternative names
Vinculin, Metavinculin, MV, VCL
Recommended products
Mouse Monoclonal Vinculin antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL aa 150-350.
Alternative names
Vinculin, Metavinculin, MV, VCL
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human VCL aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P18206
- Clone number
VCL/2575
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from Bioreactor Concentrate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (ab269680), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-vcl-2575-ab269680--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109338.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (ab269680)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (ab269680)
Formalin-fixed, paraffin-embedded human testicular carcinoma tissue stained for Vinculin using ab269680 at 2 μg/ml in immunohistochemical analysis.
![Western blot - Anti-Vinculin antibody [VCL/2575] (ab269680), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-vcl-2575-ab269680--western-blot-img37028.jpg "Western blot - Anti-Vinculin antibody [VCL/2575] (ab269680)")
Western blot - Anti-Vinculin antibody [VCL/2575] (ab269680)
All lanes: Western blot - Anti-Vinculin antibody [VCL/2575] (ab269680) at 2 µg/mL
Lane 1: U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2: THP-1 (human monocytic leukemia cell line) cell lysate
Predicted band size: 124 kDa
![Protein Array - Anti-Vinculin antibody [VCL/2575] (ab269680), expandable thumbnail](https://content.abcam.com/products/images/vinculin-antibody-vcl-2575-ab269680--protein-array-img141660.jpg "Protein Array - Anti-Vinculin antibody [VCL/2575] (ab269680)")
Protein Array - Anti-Vinculin antibody [VCL/2575] (ab269680)
Protein Array containing more than 19,000 full-length human proteins using ab269680.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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