Anti-Vinculin antibody [VCL/2575] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (AB269690)

Formalin/PFA-fixed paraffin-embedded human testicular carcinoma tissue stained for Vinculin using ab269680 at 2 μg/ml in immunohistochemistry.

This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

• WB

Supplier Data

Western blot - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (AB269690)

This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

All lanes:

Western blot - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690) at 2 µg/mL

Lane 1:

A431 (Human epidermoid carcinoma cell line) cell lysate

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Predicted band size: 124 kDa

false

• Protein Array

Supplier Data

Protein Array - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (AB269690)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab269680.- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).