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Mouse Monoclonal Vinculin antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL aa 150-350.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (AB269680), expandable thumbnail
  • Western blot - Anti-Vinculin antibody [VCL/2575] - Loading Control (AB269680), expandable thumbnail
  • Protein Array - Anti-Vinculin antibody [VCL/2575] - Loading Control (AB269680), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human VCL aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P18206

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBProtein ArrayIHC-P
Human
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

6 products for Alternative Product

Target data

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Alternative names

Recommended products

Mouse Monoclonal Vinculin antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VCL aa 150-350.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human VCL aa 150-350. The exact immunogen used to generate this antibody is proprietary information. Database link P18206
Clone number
VCL/2575
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from Bioreactor Concentrate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (ab269680), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (ab269680)

    Formalin-fixed, paraffin-embedded human testicular carcinoma tissue stained for Vinculin using ab269680 at 2 μg/ml in immunohistochemical analysis.

  • Western blot - Anti-Vinculin antibody [VCL/2575] - Loading Control (ab269680), expandable thumbnail

    Western blot - Anti-Vinculin antibody [VCL/2575] - Loading Control (ab269680)

    Vinculin Western blot staining using mouse Anti-Vinculin antibody

    All lanes: Western blot - Anti-Vinculin antibody [VCL/2575] - Loading Control (ab269680) at 2 µg/mL

    Lane 1: U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate

    Lane 2: THP-1 (human monocytic leukemia cell line) cell lysate

    Predicted band size: 124 kDa

  • Protein Array - Anti-Vinculin antibody [VCL/2575] - Loading Control (ab269680), expandable thumbnail

    Protein Array - Anti-Vinculin antibody [VCL/2575] - Loading Control (ab269680)

    Protein Array containing more than 19,000 full-length human proteins using ab269680.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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