AB242068

violetFluor™ 450 Anti-CD127 antibody [A7R34]

Name: Anti-CD127 violetFluor™ 450 antibody [A7R34] (ab242068) IgG2a | Abcam
Brand: Abcam Limited
SKU: AB242068
Price: 430 USD
Availability: InStock

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(1 Publication)

Rat Monoclonal CD127 antibody - conjugated to violetFluor™ 450. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD127, Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, IL7R

1 Images

Flow Cytometry - violetFluor™ 450 Anti-CD127 antibody [A7R34] (AB242068)

Flow Cyt

Supplier Data

Flow Cytometry - violetFluor™ 450 Anti-CD127 antibody [A7R34] (AB242068)

Flow cytometric analysis of mouse C57Bl/6 splenocyte cells staining CD127 using FITC Anti-Mouse CD3 and 0.5 μg ab242068 (right panel) or 0.5 μg violetFluor^™ 450 rat IgG2a isotype control (left panel).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

A7R34

Isotype

IgG2a

Light chain type

kappa

Conjugation

violetFluor™ 450

Excitation/Emission

Ex: 405nm, Em: 450nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>0.5 μg: The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD127 also known as IL-7R alpha or IL-7Rα is a protein found on the surface of cells involved in key immune functions. The CD127 protein has an approximate mass of 55-60 kDa. It is a component of the interleukin-7 receptor which also includes the common gamma chain. You can find CD127 expressed on various immune cells like T cells B cells and dendritic cells. As a marker CD127 is an important indicator of T cell maturity and function. Researchers often target CD127 in studies using antibodies in assay formats like ELISA to measure its expression level and function.

Biological function summary

CD127 plays an important role in regulating lymphocyte development and survival. It is part of the IL-7R complex partnering with IL-7 for signal transduction that promotes cell proliferation and survival. This interaction is essential for the development and homeostasis of T cells and B cells. In the absence of proper CD127 signaling lymphocytes may suffer from poor survival impacting overall immune competence. The IL-7-CD127 interaction informs researchers about mechanisms of immune modulation and resilience.

Pathways

CD127 participates in the JAK/STAT signaling pathway which is pivotal for transmitting information from extracellular signals to the cell nucleus influencing gene expression. This pathway plays a vital role in immune cell response. When IL-7 binds to the IL-7R complex on CD127 it initiates a cascade that involves proteins like JAK1 and STAT5. These proteins mediate responses necessary for cell proliferation and differentiation within immune system networks.

CD127 has implications in conditions like severe combined immunodeficiency (SCID) and autoimmune diseases. In SCID mutations or defects in CD127 cause impaired T cell development leading to compromised immune function. Additionally CD127 dysregulation can contribute to autoimmune disorders by affecting T regulatory cells creating an imbalance in immune response. Ocrevus a monoclonal antibody therapy used for multiple sclerosis indirectly relates to CD127 by targeting the B cells which rely on IL-7R signaling mechanisms for survival and function. Researchers continue to investigate CD127 to better understand its role in immune-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

See full target information IL7R

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 16:867-883 PubMed37915320

2023

B7 Induces Apoptosis in Colorectal Cancer Cells by Regulating the Expression of Caspase-3 and Inhibits Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Zhang,Fengxi Li,Rong Li,Nan Zhao,Dianfeng Liu,Yuelin Xu,Lei Wang,Dongxu Wang,Ruihong Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

violetFluor™ 450 Anti-CD127 antibody [A7R34]

Flow Cytometry - violetFluor™ 450 Anti-CD127 antibody [A7R34] (AB242068)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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