AB241934

violetFluor™ 450 Anti-CD19 antibody [1D3]

Lab Essentials
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(1 Publication)

Rat Monoclonal CD19 antibody - conjugated to violetFluor™ 450. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD19, B-lymphocyte antigen CD19, Differentiation antigen CD19, Cd19

1 Images

Flow Cytometry - violetFluor™ 450 Anti-CD19 antibody [1D3] (AB241934)

Flow Cyt

Supplier Data

Flow Cytometry - violetFluor™ 450 Anti-CD19 antibody [1D3] (AB241934)

Flow cytometric analysis of C57Bl/6 splenocytes stained for CD19 with 0.5 μg ab241934 (solid line) or 0.5 μg violetFluor™ 450 Rat IgG2a isotype control (dashed line).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

1D3

Isotype

IgG2a

Light chain type

kappa

Conjugation

violetFluor™ 450

Excitation/Emission

Ex: 405nm, Em: 450nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.5 μg.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

ab241934 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 20101619, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 7542548, PubMed : 7543183, PubMed : 9317126). Required for normal differentiation of B-1 cells (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 12387743, PubMed : 7542548, PubMed : 9317126). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183).

See full target information Cd19

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 22:1900-1920 PubMed37603831

2023

is a key factor regulating reticulophagy-mediated ferroptosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Bi,Qianqian Lu,Xiaohong Pan,Fenglin Dong,Yejia Hu,Zongzhen Xu,Peng Xiu,Zhiqian Liu,Jie Li

View all publications

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