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AB242009

violetFluor™ 450 Anti-CD4 antibody [GK1.5]

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(1 Publication)

Rat Monoclonal CD4 antibody - conjugated to violetFluor™ 450. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

1 Images
Flow Cytometry - violetFluor™ 450 Anti-CD4 antibody [GK1.5] (AB242009)
  • Flow Cyt

Supplier Data

Flow Cytometry - violetFluor™ 450 Anti-CD4 antibody [GK1.5] (AB242009)

Flow cytometric analysis of C57Bl/6 splenocytes stained for CD8CD4 with 0.25 μg ab242009 (solid line) or 0.25 μg violetFluor™ 450 Rat IgG2b isotype control (dashed line).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

GK1.5

Isotype

IgG2b

Light chain type

kappa

Conjugation

violetFluor™ 450

Excitation/Emission

Ex: 405nm, Em: 450nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Syrian hamster": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

If used together, the GK1.5 antibody and an alternative antibody, anti-Mouse CD4 clone RM4-5, will compete for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
See full target information Cd4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 12:702218 PubMed34385921

2021

Neuropathic Injury-Induced Plasticity of GABAergic System in Peripheral Sensory Ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Caixue Wang,Han Hao,Kaitong He,Yating An,Zeyao Pu,Nikita Gamper,Hailin Zhang,Xiaona Du
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