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Rat Monoclonal CD4 antibody - conjugated to violetFluor™ 450. Suitable for Flow Cyt and reacts with Mouse samples.

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Images

Flow Cytometry - violetFluor™ 450 Anti-CD4 antibody [GK1.5] (AB242009), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Rat
Conjugation
violetFluor™ 450
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested
Syrian hamster
Predicted

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Predicted
Predicted

Species
Syrian hamster
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

8 products for Alternative Product

Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

Alternative names

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

Recommended products

Rat Monoclonal CD4 antibody - conjugated to violetFluor™ 450. Suitable for Flow Cyt and reacts with Mouse samples.

Key facts

Isotype
IgG2b
Conjugation
violetFluor™ 450
Form
Liquid
Clonality
Monoclonal
Clone number
GK1.5
Purification technique
Affinity purification
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

If used together, the GK1.5 antibody and an alternative antibody, anti-Mouse CD4 clone RM4-5, will compete for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

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Product promise

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1 product image

  • Flow Cytometry - violetFluor™ 450 Anti-CD4 antibody [GK1.5] (ab242009), expandable thumbnail

    Flow Cytometry - violetFluor™ 450 Anti-CD4 antibody [GK1.5] (ab242009)

    Flow cytometric analysis of C57Bl/6 splenocytes stained for CD8CD4 with 0.25 μg ab242009 (solid line) or 0.25 μg violetFluor™ 450 Rat IgG2b isotype control (dashed line).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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