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AB253061

violetFluor™ 450 Anti-CD45R antibody [RA3-6B2]

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(1 Publication)

Anti-CD45R antibody [RA3-6B2] conjugated to violetFluor™ 450 - suitable for flow cytometry, and reacts with mouse samples. Clone [RA3-6B2] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - violetFluor™ 450 Anti-CD45R antibody [RA3-6B2] (AB253061)
  • Flow Cyt

Supplier Data

Flow Cytometry - violetFluor™ 450 Anti-CD45R antibody [RA3-6B2] (AB253061)

Flow cytometric analysis of mouse C57Bl/6 splenocytes stained for CD45R using ab253061 at 0.5 μg per reaction (blue) or a rat IgG2a violetFluor™ 450 isotype control (black dashed line).

  • Unconjugated

    Anti-CD45R antibody [RA3-6B2]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD45R antibody [RA3-6B2]

  • 519 FITC

    FITC Anti-CD45R antibody [RA3-6B2]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD45R antibody [RA3-6B2]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD45R antibody [RA3-6B2]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RA3-6B2

Isotype

IgG2a

Light chain type

kappa

Conjugation

violetFluor™ 450

Excitation/Emission

Ex: 405nm, Em: 450nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use at 0.5 μg per reaction.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45R also known as Leukocyte Common Antigen or LCA is a protein that plays a significant role in immune cell function. It is an isoform of the larger protein CD45 which includes several alternatively spliced variants. CD45R is a transmembrane protein tyrosine phosphatase with a mass ranging from 180 to 220 kDa depending on its isoform. This protein is primarily expressed on hematopoietic cells including T cells B cells and other leukocytes. It functions mechanically by modulating signaling thresholds essential for lymphocyte development and activation.
Biological function summary

CD45R influences signaling pathways critical for immune response regulation. It does not form a complex by itself but interacts with other membrane receptors and cytoplasmic kinases to mediate signal transduction. The protein adjusts the activity of Src family kinases through its phosphatase activity impacting cell-cell communication and responsive behavior to antigens. CD45R is involved in maintaining a functional immune surveillance system particularly ensuring the adequate responsiveness of lymphocytes.

Pathways

CD45R's phosphatase activity is integral to the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways. This activity regulates Lck and Fyn kinases within these pathways contributing to proper lymphocyte activation and development. Any disruption in CD45R function can affect these pathways potentially impacting proteins like ZAP-70 and Syk which are essential for signal transduction downstream of the TCR and BCR respectively.

CD45R has been implicated in conditions such as severe combined immunodeficiency (SCID) and autoimmune diseases like multiple sclerosis. Its malfunction or aberrant expression can cause improper immune system activation or suppression. In autoimmune contexts CD45R's interaction with other proteins like CTLA-4 may become dysregulated leading to immune system attacks on the body's tissues. Understanding these connections helps in developing therapeutic strategies targeting immune dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC

Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:68 PubMed39917001

2025

N-acetyltransferase 10 regulates UNC-51-like kinase 1 to reduce tubular cell injury and kidney stone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Le Wang,Jinjing Huang,Lei Song,Ben Ke
View all publications
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