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AB253070

violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]

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(1 Publication)

Rat Monoclonal Ly6g antibody - conjugated to violetFluor™ 450. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

1 Images
Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (AB253070)
  • Flow Cyt

Supplier Data

Flow Cytometry - violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5] (AB253070)

Flow cytometric analysis of mouse C57Bl/6 bone marrow cells stained for Ly6g using ab253070 at 0.06 μg per reaction (blue) or a rat IgG2b violetFluor™ 450 isotype control (black dashed line).

  • Unconjugated

    Anti-Ly6g + Ly6c antibody [RB6-8C5]

  • redFluor™ 710

    redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]

  • 578 PE

    PE Anti-Ly6g antibody [RB6-8C5]

  • 660 APC

    APC Anti-Ly6g antibody [RB6-8C5]

  • 778 PE/Cy7®

    PE/Cy7® Anti-Ly6g antibody [RB6-8C5]

  • 519 FITC

    FITC Anti-Ly6g antibody [RB6-8C5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RB6-8C5

Isotype

IgG2b

Light chain type

kappa

Conjugation

violetFluor™ 450

Excitation/Emission

Ex: 405nm, Em: 450nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

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Complementary Products

Primary Antibodies

AB319187

Alexa Fluor® 647 Anti-Ly6c antibody [EPR27220-23]

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6c antibody [EPR27220-23] (AB319187)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB307523

Alexa Fluor® 647 Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Alexa Fluor® 647 Anti-CD11b antibody [EPR1344] (AB307523)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use at 0.06 μg per reaction.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from tissue culture supernatant. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Ly6g
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed39595137

2024

and Are Appropriate Reference Genes for Splenic Neutrophils Isolated from Healthy or Tumor-Bearing Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Khetam Sounbuli,Ludmila A Alekseeva,Aleksandra V Sen'kova,Innokenty A Savin,Marina A Zenkova,Nadezhda L Mironova
View all publications
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Product promise

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