AB8556

Anti-VIP antibody

Name: Anti-VIP antibody (ab8556) | Abcam
Brand: Abcam Limited
SKU: AB8556

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(20 Publications)

Rabbit Polyclonal VIP antibody. Suitable for ICC, IP, WB, IHC-FoFr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Full Length Protein corresponding to Human VIP.

View Alternative Names

VIP peptides, VIP

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-FoFr, WB, IP, IHC-P, ICC

applications

Immunogen

Full Length Protein corresponding to Human VIP. The exact immunogen used to generate this antibody is proprietary information.

P01282

Specificity

This antibody reacts with a 3 kD vasointestinal peptide (VIP) localized in nerve fibers in the central and peripheral nervous system. The VIP producing tumors are usually neuroblastomas of endocrine tumors in the pancreas.

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Reactivity data

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Product details

This antibody detects ganglion cells in both the superficial and deep plexus of the wall of the small bowel in human.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

After solid-phase absorption this antibody is filtered through a 0.22 µm filter, vialed and lyophilized.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vasoactive Intestinal Peptide (VIP) also known as VIP or Alexa VIP is a 28-amino acid neuropeptide with a molecular mass of approximately 3326 Da. VIP is broadly expressed in both the central and peripheral nervous systems. It plays a mechanical role as a neurotransmitter and neuromodulator impacting various physiological functions such as vasodilation regulation of prolactin secretion and modulation of intestinal motility. By engaging in these processes VIP affects multiple bodily systems and acts at many sites within the human body.

Biological function summary

This protein influences immune response smooth muscle activity and circadian rhythm. VIP operates independently and as part of a complex in the body interacting with specific receptor types on the cellular surface like VPAC1 and VPAC2 receptors which are part of the G-protein coupled receptors family. VIP’s role as a regulator of immune response includes modulation of cytokine production and influencing lymphocyte activity implicating it as a significant player in homeostasis.

Pathways

A well-known function of VIP involves its participation in the cAMP signaling pathway which contributes to various cellular responses like neurotransmitter release and neuropeptide-mediated pathways influencing endocrine systems. VIP's activity in these pathways connects it to other proteins such as adenylate cyclase which it influences to increase cAMP levels further affecting processes such as synaptic transmission and the regulation of circadian rhythms. Through these pathways VIP affects physiological processes across the nervous and endocrine systems coordinating different aspects of bodily function.

VIP has notable associations with conditions such as inflammatory bowel disease (IBD) and asthma. The modulation of inflammatory processes by VIP impacts these diseases by influencing immune responses which can either exacerbate or alleviate symptoms. In IBD VIP interacts with elements of the immune system reducing inflammation and helping maintain mucosal integrity. In asthma VIP can alter airway inflammation and smooth muscle contraction through its pathways connected to the immune system and smooth muscle tissues interacting with proteins like cytokines that play roles in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.. PHM and PHV also cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin.

See full target information VIP

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 16:1540465 PubMed40177363

2025

Aging-related impairment of neurogenic chloride secretion in human colon mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiyun Wang,Jing He,Pengcheng Yang,Tao Bai,Jun Song,Xiaohua Hou,Lei Zhang

Cellular and molecular gastroenterology and hepatology 19:101439 PubMed39667579

2024

Elevated Bile Acids Induce Circadian Rhythm Sleep Disorders in Chronic Liver Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Zhou,Min Yan,Qin Luo,Wen Qiu,Yu-Ru Guo,Xiao-Qing Guo,Hong-Bin Yu,Jing-Ru Huo,Yan-Lin Feng,De-Ping Wang,Teng Sun,Kai-Fang Wang,Jian-Yun Shi,Xuan Shang,Mei-Na Wu,Lin Wang,Ji-Min Cao

Frontiers in microbiology 15:1327630 PubMed38601933

2024

Electroacupuncture regulates gut microbiota to reduce depressive-like behavior in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Junying Wang,Haohan Zhu,Xingke Song,Jun Zhao,Jianliang Zhang,Jinling Zhang,Shaoyuan Li,Peijing Rong

The European journal of neuroscience 59:1993-2015 PubMed38382910

2024

Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Azize Yasemin Goksu,Fatma Gonca Kocanci,Ersin Akinci,Devrim Demir-Dora,Fulya Erendor,Salih Sanlioglu,Hilmi Uysal

Cells 12: PubMed37681909

2023

Human Mast Cells Upregulate Cathepsin B, a Novel Marker of Itch in Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Peter W West,Chiara Tontini,Haris Atmoko,Orsolya Kiss,Terence Garner,Rajia Bahri,Richard B Warren,Christopher E M Griffiths,Adam Stevens,Silvia Bulfone-Paus

Nature neuroscience 26:820-829 PubMed37024573

2023

Prefrontal engrams of long-term fear memory perpetuate pain perception.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Stegemann,Sheng Liu,Oscar Andrés Retana Romero,Manfred Josef Oswald,Yechao Han,Carlo Antonio Beretta,Zheng Gan,Linette Liqi Tan,William Wisden,Johannes Gräff,Rohini Kuner

Journal of digestive diseases 21:711-723 PubMed33405317

2020

Role of free radical scavenging activity of vasoactive intestinal peptide in the attenuation of mitochondrial dysfunction to ameliorate dextran sulphate sodium-induced colitis in mice: Implications in ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Bengaluru Chandraiah Spoorthi,Sunil S More,Santhekadur Annappa Gautham,Shashwati Ghosh,Ishita Saha,Arpan Kumar Maiti

Gene therapy 28:130-141 PubMed32733091

2020

Lentiviral gene therapy vectors encoding VIP suppressed diabetes-related inflammation and augmented pancreatic beta-cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Fulya Erendor,Elif Ozgecan Sahin,Ahter D Sanlioglu,Mustafa Kemal Balci,Thomas S Griffith,Salih Sanlioglu

Oncogenesis 9:38 PubMed32205838

2020

Membrane metalloendopeptidase suppresses prostate carcinogenesis by attenuating effects of gastrin-releasing peptide on stem/progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chieh-Yang Cheng,Zongxiang Zhou,Meredith Stone,Bao Lu,Andrea Flesken-Nikitin,David M Nanus,Alexander Yu Nikitin

Reproduction (Cambridge, England) 157:475-484 PubMed30817320

2019

VIP activates primordial follicles of rat through ERK-mTOR pathway in tissue culture.

Applications

Unspecified application

Species

Unspecified reactive species

Song Li,Qi Fan,Yanqiu Xie,Haiyan Lin,Qi Qiu,Yihua Liang,Qingxue Zhang

View all publications

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