Rabbit Recombinant Monoclonal VIP antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-frozen-sections-img111000.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12601.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589)")
![Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-frozen-sections-img46555.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (AB273589)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM and PHV also cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin.
Alternative names
VIP peptides, VIP
Recommended products
Rabbit Recombinant Monoclonal VIP antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Rat, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23288-43
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273589 is the carrier-free version of Anti-VIP antibody [EPR23288-43] ab272726.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Vasoactive Intestinal Peptide (VIP) also known as VIP or Alexa VIP is a 28-amino acid neuropeptide with a molecular mass of approximately 3326 Da. VIP is broadly expressed in both the central and peripheral nervous systems. It plays a mechanical role as a neurotransmitter and neuromodulator impacting various physiological functions such as vasodilation regulation of prolactin secretion and modulation of intestinal motility. By engaging in these processes VIP affects multiple bodily systems and acts at many sites within the human body.
- Biological function summary
This protein influences immune response smooth muscle activity and circadian rhythm. VIP operates independently and as part of a complex in the body interacting with specific receptor types on the cellular surface like VPAC1 and VPAC2 receptors which are part of the G-protein coupled receptors family. VIP’s role as a regulator of immune response includes modulation of cytokine production and influencing lymphocyte activity implicating it as a significant player in homeostasis.
- Pathways
A well-known function of VIP involves its participation in the cAMP signaling pathway which contributes to various cellular responses like neurotransmitter release and neuropeptide-mediated pathways influencing endocrine systems. VIP's activity in these pathways connects it to other proteins such as adenylate cyclase which it influences to increase cAMP levels further affecting processes such as synaptic transmission and the regulation of circadian rhythms. Through these pathways VIP affects physiological processes across the nervous and endocrine systems coordinating different aspects of bodily function.
- Associated diseases and disorders
VIP has notable associations with conditions such as inflammatory bowel disease (IBD) and asthma. The modulation of inflammatory processes by VIP impacts these diseases by influencing immune responses which can either exacerbate or alleviate symptoms. In IBD VIP interacts with elements of the immune system reducing inflammation and helping maintain mucosal integrity. In asthma VIP can alter airway inflammation and smooth muscle contraction through its pathways connected to the immune system and smooth muscle tissues interacting with proteins like cytokines that play roles in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-frozen-sections-img111000.jpg "Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)")
Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon tissue labeling VIP with Anti-VIP antibody [EPR23288-43] ab272726 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on enteric nerve of rat colon (PMID: 26446924) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12601.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
Immunohistochemical analysis of paraffin-embedded Human duodenum tissue labeling VIP with Anti-VIP antibody [EPR23288-43] ab272726 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on enteric nerve of human duodenum. The section was incubated with Anti-VIP antibody [EPR23288-43] ab272726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).
![Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-frozen-sections-img46555.jpg "Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)")
Immunohistochemistry (Frozen sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling VIP with Anti-VIP antibody [EPR23288-43] ab272726 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on enteric nerve of mouse colon (PMID: 26446924) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
Immunohistochemical analysis of paraffin-embedded Rat small intestine tissue labeling VIP with Anti-VIP antibody [EPR23288-43] ab272726 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on enteric nerve of rat small intestine (PMID: 7014205). The section was incubated with Anti-VIP antibody [EPR23288-43] ab272726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589), expandable thumbnail](https://content.abcam.com/products/images/vip-antibody-epr23288-43-bsa-and-azide-free-ab273589--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP antibody [EPR23288-43] - BSA and Azide free (ab273589)
Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue labeling VIP with Anti-VIP antibody [EPR23288-43] ab272726 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on enteric nerve of mouse small intestine. The section was incubated with Anti-VIP antibody [EPR23288-43] ab272726 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab272726).
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