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Rabbit Recombinant Monoclonal VIP antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-VIP antibody [EPR28121-6] (AB324196), expandable thumbnail
  • Western blot - Anti-VIP antibody [EPR28121-6] (AB324196), expandable thumbnail
  • Western blot - Anti-VIP antibody [EPR28121-6] (AB324196), expandable thumbnail
  • Western blot - Anti-VIP antibody [EPR28121-6] (AB324196), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM and PHV also cause vasodilation. PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin.

Alternative names

VIP peptides, VIP

Rabbit Recombinant Monoclonal VIP antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28121-6
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-VIP antibody [EPR28121-6] (ab324196), expandable thumbnail

    Western blot - Anti-VIP antibody [EPR28121-6] (ab324196)

    VIP Western blot staining using rabbit Anti-VIP antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 31559013).

    The identity of the higher MW band at approximately 70 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-VIP antibody [EPR28121-6] (ab324196) at 1/1000 dilution

    Lane 1: Untreated HuTu 80 (human Small intestine; Duodenum epithelial) whole cell lysate at 20 µg

    Lane 2: HuTu 80 treated with 300ng/ml BFA for 24 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 23 kDa, 24 kDa, 36 kDa

    Exposure time: 6s

  • Western blot - Anti-VIP antibody [EPR28121-6] (ab324196), expandable thumbnail

    Western blot - Anti-VIP antibody [EPR28121-6] (ab324196)

    VIP Western blot staining using rabbit Anti-VIP antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 31559013).

    The identity of the higher MW band at approximately 70 kDa is unknown.

    In Western blot, Anti-H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/50000 dilution.

    All lanes: Western blot - Anti-VIP antibody [EPR28121-6] (ab324196) at 1/1000 dilution

    Lane 1: SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Capan-1 (human pancreatic adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

    Lane 3: Capan-1 nuclear fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 23 kDa, 24 kDa, 36 kDa, 15 kDa

    Exposure time: 6s

  • Western blot - Anti-VIP antibody [EPR28121-6] (ab324196), expandable thumbnail

    Western blot - Anti-VIP antibody [EPR28121-6] (ab324196)

    VIP Western blot staining using rabbit Anti-VIP antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: SK-MEL-2, HepG2.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 31559013).

    The identity of the higher MW band at approximately 70 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-VIP antibody [EPR28121-6] (ab324196) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 4: SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg

    Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 24 kDa, 23 kDa, 36 kDa

    Exposure time: 6s

  • Western blot - Anti-VIP antibody [EPR28121-6] (ab324196), expandable thumbnail

    Western blot - Anti-VIP antibody [EPR28121-6] (ab324196)

    VIP Western blot staining using rabbit Anti-VIP antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: skin.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 31559013).

    The identity of the higher MW band at approximately 70 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-VIP antibody [EPR28121-6] (ab324196) at 1/1000 dilution

    Lane 1: Human small intestine tissue lysate at 20 µg

    Lane 2: Human stomach tissue lysate at 20 µg

    Lane 3: Human brainstem tissue lysate at 20 µg

    Lane 4: Human skin tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 23 kDa, 24 kDa, 36 kDa

    Exposure time: 6s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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