AB154046

Anti-VIP2 antibody

Name: Anti-VIP2 antibody (ab154046) | Abcam
Brand: Abcam Limited
SKU: AB154046
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal VIP2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPIP5K2 aa 1-350.

View Alternative Names

HISPPD1, KIAA0433, VIP2, PPIP5K2, Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2, Diphosphoinositol pentakisphosphate kinase 2, Histidine acid phosphatase domain-containing protein 1, InsP6 and PP-IP5 kinase 2, VIP1 homolog 2, hsVIP2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP2 antibody (AB154046)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP2 antibody (AB154046)

Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue, labeling VIP2 with ab154046 at 1/500 dilution.

Unknown

Western blot - Anti-VIP2 antibody (AB154046)

5% SDS PAGE

All lanes:

Western blot - Anti-VIP2 antibody (ab154046) at 1/3000 dilution

Lane 1:

Raji whole cell lysate at 30 µg

Lane 2:

K562 whole cell lysate at 30 µg

Predicted band size: 140 kDa

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CiteAb

Western blot - Anti-VIP2 antibody (AB154046)

VIP2 western blot using anti-VIP2 antibody ab154046. Publication image and figure legend from Gu, C., Wilson, M. S., et al., 2016, PLoS One, PubMed 27788189.

ab154046 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154046 please see the product overview.

Comparisons of HCT116NIH and HCT116UCL cells : expression of IP6Ks and PPIP5Ks, capacity to dephosphorylate InsP8, cell growth, and phalloidin staining.The following analyses of HCT116NIH and HCT116UCL cells were performed : Panel A, Western analyses of IP6Ks and PPIP5Ks. Complete gels, and procedures used to validate the antibodies, are described in S1 and S2 Figs. Panel B, quantitative RT-PCR analysis of expression of IP6K1, IP6K2 and IP6K3. Panel C, HPLC analysis of 1 μM [3H]InsP8 dephosphorylation by 70 μg cell lysates in 100 μl medium. Panel D, counting of cell growth for the indicated number of days. Panel E, labeling of the actin cytoskeleton with FITC-phalloidin. Hoechst was used as a nuclear stain.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PPIP5K2 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

O43314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The vasointestinal peptide receptor type 2 (VIP2) also known as VIPR2 is a transmembrane G protein-coupled receptor with a mass of approximately 52 kDa. VIP2 mediates its effects through the binding of vasoactive intestinal peptide (VIP). It predominantly expresses in the central nervous system including the hippocampus and cerebral cortex and some peripheral tissues such as the gastrointestinal tract. VIPR2 plays an important role in transmitting extracellular signals into intracellular responses.

Biological function summary

Within different tissues VIP2 functions in regulating circadian rhythms immune responses and neuroprotection. VIP2 serves as an important component of a receptor complex that interacts with other neuropeptides and neurotransmitters. Its activity influences the modulation of neurophysiological processes such as neurotransmission and hormone secretion. The receptor's ability to initiate cAMP production following VIP binding is central to its biological activity.

Pathways

VIP2 is involved in critical signaling pathways like the cAMP-PKA signaling pathway and the circadian rhythm regulation pathway. These pathways allow the integration of environmental signals to maintain homeostasis and organism health. In the cAMP-PKA pathway VIP2 interacts closely with related receptors such as VPAC1 and VPAC2 sharing similar downstream effects that ultimately impact gene expression and cellular communication.

VIP2 has been associated with schizotypal personality disorder and certain autoimmune conditions. There is evidence suggesting VIP2 variations may contribute to altered neurodevelopment processes linked to psychiatric conditions. The receptor's dysfunction impacts neuroinflammation where it might connect with proteins like cytokines and chemokines that mediate immune responses. Understanding VIP2's role could lead to potential therapeutic targets for these challenging conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4 (PubMed : 17690096, PubMed : 17702752, PubMed : 21222653, PubMed : 29590114). PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation (PubMed : 17690096, PubMed : 17702752, PubMed : 21222653, PubMed : 29590114). Phosphorylates inositol hexakisphosphate (InsP6) at position 1 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4 (PubMed : 17690096, PubMed : 17702752). Alternatively, phosphorylates PP-InsP5 at position 1, produced by IP6Ks from InsP6, to produce (PP)2-InsP4 (PubMed : 17690096, PubMed : 17702752). Required for normal hearing (PubMed : 29590114).

See full target information PPIP5K2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 117:3568-3574 PubMed32019887

2020

Control of XPR1-dependent cellular phosphate efflux by InsP is an exemplar for functionally-exclusive inositol pyrophosphate signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyao Li,Chunfang Gu,Sarah Hostachy,Soumyadip Sahu,Christopher Wittwer,Henning J Jessen,Dorothea Fiedler,Huanchen Wang,Stephen B Shears

PloS one 11:e0165286 PubMed27788189

2016

Inositol Pyrophosphate Profiling of Two HCT116 Cell Lines Uncovers Variation in InsP8 Levels.

Applications

Species

Human

Chunfang Gu,Miranda S C Wilson,Henning J Jessen,Adolfo Saiardi,Stephen B Shears

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VIP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP2 antibody (AB154046)

Western blot - Anti-VIP2 antibody (AB154046)

Western blot - Anti-VIP2 antibody (AB154046)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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