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AB224227

Anti-VIP36 antibody

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(1 Publication)

Rabbit Polyclonal VIP36 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LMAN2 aa 1-200.

View Alternative Names

C5orf8, LMAN2, Vesicular integral-membrane protein VIP36, Glycoprotein GP36b, Lectin mannose-binding 2, Vesicular integral-membrane protein 36, VIP36

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP36 antibody (AB224227)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP36 antibody (AB224227)

Paraffin-embedded human lungs stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows weak to moderate cytoplasmic positivity in macrophages.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP36 antibody (AB224227)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP36 antibody (AB224227)

Paraffin-embedded human skeletal muscle stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows no positivity in myocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP36 antibody (AB224227)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIP36 antibody (AB224227)

Paraffin-embedded human testis stained for VIP36 using ab224227 at 1/50 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in cells of the seminiferous duct.

Western blot - Anti-VIP36 antibody (AB224227)
  • WB

Supplier Data

Western blot - Anti-VIP36 antibody (AB224227)

All lanes:

Western blot - Anti-VIP36 antibody (ab224227) at 1/100 dilution

Lane 1:

Vector only transfected HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing VIP36 (co-expressed with a C terminal myc-DDK tag), cell lysate

Predicted band size: 40 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LMAN2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q12907

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VIP36 also known as vesicular integral-membrane protein 36 operates as a lectin involved in glycoprotein transport within the cell. It has a mass of approximately 36 kDa. Scientists observe its expression mainly in the liver and testis where it localizes to the Golgi apparatus and endoplasmic reticulum. VIP36 functions to select specific N-linked glycans facilitating their sorting and transport essential for proper protein localization and function.
Biological function summary

Vesicular integral-membrane protein 36 contributes to the quality control of glycoproteins within the secretory pathway. While not forming part of a larger complex VIP36 plays a critical role in recognizing high-mannose-type glycoproteins which are critical for cell-cell communication and adhesion. This function helps maintain cellular homeostasis and impacts various cellular activities reflecting the protein's importance in cellular processes.

Pathways

Glycoprotein transport and sorting rely on VIP36 which interacts with pathways like the secretory pathway and vesicle trafficking. The protein impacts the dynamics of protein glycosylation fundamental for the correct functioning of proteins like E-cadherin. By ensuring glycoproteins reach their proper destinations within the cell VIP36 plays an important role in maintaining cellular architecture and facilitating important signaling processes.

Disruptions in VIP36 functionality might contribute to disorders like liver cirrhosis and testicular malfunction. Problems in its glycoprotein sorting capability can affect proteins such as alpha-fetoprotein potentially altering normal cellular function and leading to disease. Researchers continue to investigate VIP36's role in these disorders aiming to understand how alterations in this protein could offer insights into therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans (By similarity).
See full target information LMAN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 173:634-648.e12 PubMed29606356

2018

Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmei Han,Qiuyan Liu,Jin Hou,Yan Gu,Yi Zhang,Zhubo Chen,Jia Fan,Weiping Zhou,Shuangjian Qiu,Yonghong Zhang,Tao Dong,Ning Li,Zhengping Jiang,Ha Zhu,Qian Zhang,Yuanwu Ma,Lianfeng Zhang,Qingqing Wang,Yizhi Yu,Nan Li,Xuetao Cao
View all publications
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Product promise

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