Anti-Viperin antibody [MaP.VIP]

• IP

Lab

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (AB107359)

Viperin was immunoprecipitated from 0.35 mg KG-1a (human bone marrow myeloblast) whole cell lysate with ab107359 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (ab107359) at 1/50 dilution

Lane 1:

KG-1a (human bone marrow myeloblast) whole cell lysate (Input) at 10 µg

Lane 2:

KG-1a (human bone marrow myeloblast) whole cell lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/en-us/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of ab107359 in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Viperin antibody [MaP.VIP] (AB107359)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) labelling Viperin with ab107359 at 1/500 dilution followed by secondary antibody ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (green).

ab202272 Recombinant Alexa Fluor^® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker was used as a counterstain at a 1/200 dilution (magenta).

Confocal image showing cytoplasmic staining in Raw264.7 cells (shown in green) treated with 100 ng/ml LPS (lipopolysaccharides) and 100 U/ml IFN gamma for 4 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IP

Lab

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (AB107359)

Viperin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab107359 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (ab107359) at 1/50 dilution

Lane 1:

Mouse spleen tissue lysate (Input) at 10 µg

Lane 2:

Mouse spleen tissue lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/en-us/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of ab107359 in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

• WB

Lab

Western blot - Anti-Viperin antibody [MaP.VIP] (AB107359)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ab181602 was used as a GAPDH loading control at a 1/200000 dilution.

Low expression : HepG2
Negative control : SW480.

All lanes:

Western blot - Anti-Viperin antibody [MaP.VIP] (ab107359) at 1/1000 dilution

Lane 1:

HCC1937 (human mammary gland epithelial cell) whole cell lysate at 20 µg

Lane 2:

Calu-3 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 7:

RAW264.7 treated with 100ng/ml LPS and 100U/ml IFN gamma for 4h whole cell lysate at 20 µg

Lane 8:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 9:

THP-1 treated with 100ng/ml LPS and 100U/ml IFN gamma for 4h whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-Viperin antibody [MaP.VIP] (AB107359)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ab181602 was used as a GAPDH loading control at a 1/200000 dilution.

Low expression : Heart.

All lanes:

Western blot - Anti-Viperin antibody [MaP.VIP] (ab107359) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 42 kDa

false

Exposure time: 10s