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AB326612

Anti-Viperin antibody [MaP.VIP] - BSA and Azide free

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Mouse Recombinant Monoclonal Viperin antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Rsad2 aa 50 to C-terminus.

View Alternative Names

CIG5, RSAD2, S-adenosylmethionine-dependent nucleotide dehydratase RSAD2, SAND, Cytomegalovirus-induced gene 5 protein, Radical S-adenosyl methionine domain-containing protein 2, Viperin

5 Images
Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)
  • IP

Lab

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Viperin was immunoprecipitated from 0.35 mg KG-1a (human bone marrow myeloblast) whole cell lysate with ab107359 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

Lane 1 : KG-1a (human bone marrow myeloblast) whole cell lysate
Lane 2 : ab107359 IP in KG-1a (human bone marrow myeloblast) whole cell lysate
Lane 3 : Mouse IgG2a, kappaIsotype control (ab18413) instead of ab107359 in KG-1a whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 93 seconds.

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

KG-1a (human bone marrow myeloblast) whole cell lysate (Input) at 10 µg

Lane 2:

KG-1a (human bone marrow myeloblast) whole cell lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/en-us/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of <a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

Immunocytochemistry/ Immunofluorescence - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Viperin with ab107359 at 1/500 (1.966 μg/ml) dilution , followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in Raw264.7 cells (shown in green) treated with 100 ng/ml LPS (lipopolysaccharides) and 100 U/ml IFN gamma for 4 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)
  • IP

Lab

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Viperin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab107359 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab107359 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.

Lane 1 : Mouse spleen tissue lysate
Lane 2 : ab107359 IP in Mouse spleen tissue lysate
Lane 3 : Mouse IgG2a, kappaIsotype control (ab18413) instead of ab107359 in mouse spleen tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 93 seconds.

All lanes:

Immunoprecipitation - Anti-Viperin antibody [MaP.VIP] (<a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/50 dilution

Lane 1:

Mouse spleen tissue lysate (Input) at 10 µg

Lane 2:

Mouse spleen tissue lysate (+)

Lane 3:

Mouse IgG2a, kappaIsotype control (<a href='/en-us/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of <a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a> in KG-1a whole cell lysate (-)

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 93s

Western blot - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)
  • WB

Lab

Western blot - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HepG2

Negative control : SW480

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Viperin antibody [MaP.VIP] (<a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/1000 dilution

Lane 1:

HCC1937 (human mammary gland epithelial cell) whole cell lysate at 20 µg

Lane 2:

Calu-3 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 7:

RAW264.7 treated with 100ng/ml LPS and 100U/ml IFN gamma for 4h whole cell lysate at 20 µg

Lane 8:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 9:

THP-1 treated with 100ng/ml LPS and 100U/ml IFN gamma for 4h whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s

Western blot - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)
  • WB

Lab

Western blot - Anti-Viperin antibody [MaP.VIP] - BSA and Azide free (AB326612)

This data was developed using ab107359, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Viperin antibody [MaP.VIP] (<a href='/en-us/products/primary-antibodies/viperin-antibody-mapvip-ab107359'>ab107359</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 42 kDa

false

Exposure time: 10s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MaP.VIP

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human, Mouse

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Rsad2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8CBB9

Specificity

Unsuitable for human ICC/IF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab326612 is the carrier-free version of ab107359

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon (PubMed : 31812350). Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism (PubMed : 29925952, PubMed : 30872404). In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication (PubMed : 29925952). Therefore, inhibits a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), zika virus, and human immunodeficiency virus (HIV-1) (PubMed : 29925952, PubMed : 30587778, PubMed : 30934824, PubMed : 31921110). Also promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6 (PubMed : 30872404). Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.
See full target information RSAD2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com