Anti-VIPR2 antibody [HL3625] - BSA and Azide free

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

ab325876 detects VPAC2 protein by immunohistochemical analysis. Sample : Frozen-sectioned mouse pancreas. Green : VPAC2 stained by ab325876 diluted at 1 : 250. Red : Vimentin antibody diluted at 1 : 1000. Blue : Hoechst 33342 staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

ab325876 detects VPAC2 protein at suprachiasmatic nucleus by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. VPAC2 stained by ab325876 diluted at 1 : 200. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

ab325876 detects VPAC2 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse pancreas. VPAC2 stained by ab325876 diluted at 1 : 200. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

ab325876 detects VPAC2 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse small intestine. VPAC2 stained by ab325876 diluted at 1 : 200. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

ab325876 detects VPAC2 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. VPAC2 stained by ab325876 diluted at 1 : 200. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

ab325876 detects VPAC2 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. VPAC2 stained by ab325876 diluted at 1 : 200. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

• WB

Supplier Data

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

Unboiled RMS 13 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325876 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (ab325876) at 1/1000 dilution

Lane 1:

Unboiled RMS 13 whole cell extracts at 30 µg

Lane 2:

Unboiled RMS 13 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

Boiled and unboiled rat tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325876 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (ab325876) at 1/1000 dilution

Lane 1:

Boiled rat stomach tissue extracts at 50 µg

Lane 2:

Unboiled rat stomach tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

Unboiled mouse tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325876 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (ab325876) at 1/1000 dilution

Lane 1:

Unboiled mouse stomach tissue extracts at 50 µg

Lane 2:

Unboiled mouse testis tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325876 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (ab325876) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged VIPR2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged VIPR2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (AB325876)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325876 diluted at 1 : 100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-VIPR2 antibody [HL3625] - BSA and Azide free (ab325876) at 1/100000 dilution

Lanes 1 and 3:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled VIPR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled VIPR2 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false