AB246982

Anti-VIRMA/KIAA1429 antibody

Name: Anti-VIRMA/KIAA1429 antibody (ab246982) | Abcam
Brand: Abcam Limited
SKU: AB246982
Price: 655 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal VIRMA/KIAA1429 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VIRMA aa 1000-1150.

View Alternative Names

KIAA1429, MSTP054, VIRMA, Protein virilizer homolog

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-VIRMA/KIAA1429 antibody (AB246982)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VIRMA/KIAA1429 antibody (AB246982)

PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for VIRMA/KIAA1429 (green) using ab246982 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Paraffin-embedded human endometrium tissue stained for VIRMA/KIAA1429 using ab246982 at 1/20 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Paraffin-embedded human testis tissue stained for VIRMA/KIAA1429 using ab246982 at 1/20 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Paraffin-embedded human skeletal muscle tissue stained for VIRMA/KIAA1429 using ab246982 at 1/20 dilution in immunohistochemical analysis. Weak positivity, as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Paraffin-embedded human skin tissue stained for VIRMA/KIAA1429 using ab246982 at 1/20 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Paraffin-embedded human rectum tissue stained for VIRMA/KIAA1429 using ab246982 at 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VIRMA aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information.

Q69YN4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VIRMA also known as KIAA1429 is a protein with an essential role in the RNA N6-adenosine methylation process often referred to as m6A modification. It has a molecular mass around 200 kDa and is expressed widely across various tissues prominently in the brain liver and kidney. As a component of the m6A methylation machinery it associates with other proteins that determine m6A methyltransferase activity.

Biological function summary

This protein plays a significant part in mRNA modification by acting as an important component of the m6A writer complex. It contributes to the regulation of gene expression by modulating mRNA stability and translation. The m6A writer complex which includes METTL3 METTL14 and WTAP often coordinates with VIRMA to ensure proper mRNA methylation affecting cellular processes such as differentiation and proliferation. Its involvement in this complex implies its importance for mRNA metabolism and gene expression regulation.

Pathways

VIRMA significantly impacts the RNA processing and degradation pathways. Within these pathways it interacts with proteins such as METTL3 and METTL14 to influence mRNA splicing and degradation which are vital for cellular homeostasis. Moreover VIRMA is closely linked to the circadian rhythm pathway. It regulates mRNA targets affecting the rhythmic expression of clock genes underlining cellular timing mechanisms.

VIRMA is connected to cancer and metabolic disorders. Alterations in its methylation activity correlate with tumorigenesis and cancer progression particularly in liver cancer. It cooperates with proteins like METTL3 in promoting oncogenic mRNA translation leading to uncontrolled cell growth. Additionally dysregulation of RNA methylation involving VIRMA influences the metabolism contributing to disorders like obesity. This makes it an attractive target for therapeutic strategies aiming to modulate abnormal methylation patterns.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 24981863, PubMed : 29507755). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs in the 3'-UTR near the stop codon : recruits the catalytic core components METTL3 and METTL14, thereby guiding m6A methylation at specific sites (PubMed : 29507755). Required for mRNA polyadenylation via its role in selective m6A methylation : m6A methylation of mRNAs in the 3'-UTR near the stop codon correlating with alternative polyadenylation (APA) (PubMed : 29507755).

See full target information VIRMA

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 15:1397-1409 PubMed38717936

2024

KIAA1429 regulates lung adenocarcinoma proliferation and metastasis through the PI3K/AKT pathway by modulating ARHGAP30 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Tan Wang,Qilin Huai,Lei Guo,Xiaobing Wang,Jie He

Cancers 15: PubMed37958388

2023

The Mechanism of Oxymatrine Targeting miR-27a-3p/PPAR-γ Signaling Pathway through m6A Modification to Regulate the Influence on Hemangioma Stem Cells on Propranolol Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Dai,Mingke Qiu,Shenglai Zhang,Jingyu Peng,Xin Hou,Jie Liu,Feifei Li,Jingmin Ou

International journal of molecular sciences 24: PubMed37762537

2023

Synergistic Pulmonoprotective Effect of Natural Prolyl Oligopeptidase Inhibitors in In Vitro and In Vivo Models of Acute Respiratory Distress Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Stelios Zerikiotis,Panagiotis Efentakis,Danai Dapola,Anna Agapaki,Georgios Seiradakis,Nikolaos Kostomitsopoulos,Alexios-Leandros Skaltsounis,Ioulia Tseti,Filippos Triposkiadis,Ioanna Andreadou

Oncogene 38:6123-6141 PubMed31285549

2019

KIAA1429 acts as an oncogenic factor in breast cancer by regulating CDK1 in an N6-methyladenosine-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Yi Qian,Jian Gao,Xi Sun,Meng-Da Cao,Liang Shi,Tian-Song Xia,Wen-Bin Zhou,Shui Wang,Qiang Ding,Ji-Fu Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VIRMA/KIAA1429 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VIRMA/KIAA1429 antibody (AB246982)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VIRMA/KIAA1429 antibody (AB246982)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

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