Rabbit Recombinant Monoclonal VIRMA/KIAA1429 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 2 publications.
Images
![Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--western-blot-img166981.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136)")
![Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--flow-cytometry-intracellular-img165460.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136)")
![Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--western-blot-img176336.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136)")
![Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--flow-cytometry-intracellular-img173937.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136)")
![Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--western-blot-img168923.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (AB271136)")
Publications
Filter by
- The Journal of biological chemistry 299:1046772023VIRMA promotes nasopharyngeal carcinoma, tumorigenesis, and metastasis by upregulation of E2F7 in an m6A-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Qi Zheng,Zhuo-Hui Huang,Ye-Lin Liang,Wei-Hong Zheng,Cheng Xu,Zhi-Xuan Li,Na Liu,Pan-Yang Yang,Ying-Qin Li,Jun Ma,Ying Sun,Ling-Long Tang,Denghui WeiPubMed 37028765
- Cancer medicine 12:7222-72332022KIAA1429 mediates epithelial mesenchymal transition in sorafenib-resistant hepatocellular carcinoma through m6A methylation modification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Kuang,Yun Cheng,Jia Wang,Hongyan Li,Xianghong Cao,Yang WangPubMed 36420693
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC | WB | Flow Cyt (Intra) | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:24981863, PubMed:29507755). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs in the 3'-UTR near the stop codon: recruits the catalytic core components METTL3 and METTL14, thereby guiding m6A methylation at specific sites (PubMed:29507755). Required for mRNA polyadenylation via its role in selective m6A methylation: m6A methylation of mRNAs in the 3'-UTR near the stop codon correlating with alternative polyadenylation (APA) (PubMed:29507755).
Alternative names
KIAA1429, MSTP054, VIRMA, Protein virilizer homolog
Recommended products
Rabbit Recombinant Monoclonal VIRMA/KIAA1429 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24279-23
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VIRMA also known as KIAA1429 is a protein with an essential role in the RNA N6-adenosine methylation process often referred to as m6A modification. It has a molecular mass around 200 kDa and is expressed widely across various tissues prominently in the brain liver and kidney. As a component of the m6A methylation machinery it associates with other proteins that determine m6A methyltransferase activity.
- Biological function summary
This protein plays a significant part in mRNA modification by acting as an important component of the m6A writer complex. It contributes to the regulation of gene expression by modulating mRNA stability and translation. The m6A writer complex which includes METTL3 METTL14 and WTAP often coordinates with VIRMA to ensure proper mRNA methylation affecting cellular processes such as differentiation and proliferation. Its involvement in this complex implies its importance for mRNA metabolism and gene expression regulation.
- Pathways
VIRMA significantly impacts the RNA processing and degradation pathways. Within these pathways it interacts with proteins such as METTL3 and METTL14 to influence mRNA splicing and degradation which are vital for cellular homeostasis. Moreover VIRMA is closely linked to the circadian rhythm pathway. It regulates mRNA targets affecting the rhythmic expression of clock genes underlining cellular timing mechanisms.
- Associated diseases and disorders
VIRMA is connected to cancer and metabolic disorders. Alterations in its methylation activity correlate with tumorigenesis and cancer progression particularly in liver cancer. It cooperates with proteins like METTL3 in promoting oncogenic mRNA translation leading to uncontrolled cell growth. Additionally dysregulation of RNA methylation involving VIRMA influences the metabolism contributing to disorders like obesity. This makes it an attractive target for therapeutic strategies aiming to modulate abnormal methylation patterns.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--western-blot-img166981.jpg "Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)")
Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)
The observed MW are consistent with what has been described in the literature (PMID: 34490238).
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136) at 1/1000 dilution
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate at 20 µg
Lane 3: T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg
Lane 4: C2C12 (mouse myoblasts), whole cell lysate at 20 µg
Lane 5: Mouse liver cancer lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 202 kDa
Observed band size: 202 kDa
Exposure time: 26s
![Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--flow-cytometry-intracellular-img165460.jpg "Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)")
Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling VIRMA/KIAA1429 with ab271136 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--western-blot-img176336.jpg "Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)")
Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)
Lysates should be made freshly and used in WB immediately to minimize protein degradation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 202 kDa
Observed band size: 202 kDa
Exposure time: 26s
![Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--flow-cytometry-intracellular-img173937.jpg "Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)")
Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling VIRMA/KIAA1429 with ab271136 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136), expandable thumbnail](https://content.abcam.com/products/images/virma-kiaa1429-antibody-epr24279-23-ab271136--western-blot-img168923.jpg "Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)")
Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136)
Lysates should be made freshly and used in WB immediately to minimize protein degradation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (ab271136) at 1/1000 dilution
Lane 1: HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with GATA-2 siRNA 1 whole cell lysate at 20 µg
Lane 3: HeLa transfected with GATA-2 siRNA 2 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 202 kDa
Observed band size: 202 kDa
Exposure time: 59s
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