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AB289728

Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free

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Rabbit Recombinant Monoclonal VIRMA/KIAA1429 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Human samples.

View Alternative Names

KIAA1429, MSTP054, VIRMA, Protein virilizer homolog

5 Images
Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)

This data was developed using ab271136, the same antibody in a different formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling VIRMA/KIAA1429 with ab271136 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)

This data was developed using ab271136, the same antibody in a different formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling VIRMA/KIAA1429 with ab271136 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)
  • WB

Lab

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)

This data was developed using ab271136, the same antibody clone in a different buffer formulation.

The observed MW are consistent with what has been described in the literature (PMID : 34490238).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (<a href='/en-us/products/primary-antibodies/virma-kiaa1429-antibody-epr24279-23-ab271136'>ab271136</a>) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate at 20 µg

Lane 3:

T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 4:

C2C12 (mouse myoblasts), whole cell lysate at 20 µg

Lane 5:

Mouse liver cancer lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 202 kDa

Observed band size: 202 kDa

false

Exposure time: 26s

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)
  • WB

Supplier Data

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)

This data was developed using ab271136, the same antibody clone in a different buffer formulation.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (<a href='/en-us/products/primary-antibodies/virma-kiaa1429-antibody-epr24279-23-ab271136'>ab271136</a>) at 1/1000 dilution

Lane 1:

HeLa transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with GATA-2 siRNA 1 whole cell lysate at 20 µg

Lane 3:

HeLa transfected with GATA-2 siRNA 2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 202 kDa

Observed band size: 202 kDa

false

Exposure time: 59s

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)
  • WB

Supplier Data

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] - BSA and Azide free (AB289728)

This data was developed using ab271136, the same antibody clone in a different buffer formulation.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-VIRMA/KIAA1429 antibody [EPR24279-23] (<a href='/en-us/products/primary-antibodies/virma-kiaa1429-antibody-epr24279-23-ab271136'>ab271136</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 202 kDa

Observed band size: 202 kDa

false

Exposure time: 26s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24279-23

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab289728 is the carrier-free version of ab271136.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VIRMA also known as KIAA1429 is a protein with an essential role in the RNA N6-adenosine methylation process often referred to as m6A modification. It has a molecular mass around 200 kDa and is expressed widely across various tissues prominently in the brain liver and kidney. As a component of the m6A methylation machinery it associates with other proteins that determine m6A methyltransferase activity.
Biological function summary

This protein plays a significant part in mRNA modification by acting as an important component of the m6A writer complex. It contributes to the regulation of gene expression by modulating mRNA stability and translation. The m6A writer complex which includes METTL3 METTL14 and WTAP often coordinates with VIRMA to ensure proper mRNA methylation affecting cellular processes such as differentiation and proliferation. Its involvement in this complex implies its importance for mRNA metabolism and gene expression regulation.

Pathways

VIRMA significantly impacts the RNA processing and degradation pathways. Within these pathways it interacts with proteins such as METTL3 and METTL14 to influence mRNA splicing and degradation which are vital for cellular homeostasis. Moreover VIRMA is closely linked to the circadian rhythm pathway. It regulates mRNA targets affecting the rhythmic expression of clock genes underlining cellular timing mechanisms.

VIRMA is connected to cancer and metabolic disorders. Alterations in its methylation activity correlate with tumorigenesis and cancer progression particularly in liver cancer. It cooperates with proteins like METTL3 in promoting oncogenic mRNA translation leading to uncontrolled cell growth. Additionally dysregulation of RNA methylation involving VIRMA influences the metabolism contributing to disorders like obesity. This makes it an attractive target for therapeutic strategies aiming to modulate abnormal methylation patterns.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 24981863, PubMed : 29507755). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs in the 3'-UTR near the stop codon : recruits the catalytic core components METTL3 and METTL14, thereby guiding m6A methylation at specific sites (PubMed : 29507755). Required for mRNA polyadenylation via its role in selective m6A methylation : m6A methylation of mRNAs in the 3'-UTR near the stop codon correlating with alternative polyadenylation (APA) (PubMed : 29507755).
See full target information VIRMA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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