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AB308190

Anti-VIRMA/KIAA1429 antibody [HL1719]

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Rabbit Recombinant Monoclonal VIRMA/KIAA1429 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VIRMA.

View Alternative Names

KIAA1429, MSTP054, VIRMA, Protein virilizer homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-VIRMA/KIAA1429 antibody [HL1719] (AB308190)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VIRMA/KIAA1429 antibody [HL1719] (AB308190)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 min and permeabilized for detection of VIRMA protein using ab308190 at 1/500 dilution (green).

Alpha tubulin (red) is stained with alpha Tubulin antibody at 1/1000 dilution.

Western blot - Anti-VIRMA/KIAA1429 antibody [HL1719] (AB308190)
  • WB

Supplier Data

Western blot - Anti-VIRMA/KIAA1429 antibody [HL1719] (AB308190)

Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-VIRMA/KIAA1429 antibody [HL1719] (ab308190) at 1/1000 dilution

Lane 1:

K561 whole cell extract at 30 µg

Lane 2:

THP-1 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 202 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1719

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VIRMA. The exact immunogen used to generate this antibody is proprietary information.

Q69YN4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VIRMA also known as KIAA1429 is a protein with an essential role in the RNA N6-adenosine methylation process often referred to as m6A modification. It has a molecular mass around 200 kDa and is expressed widely across various tissues prominently in the brain liver and kidney. As a component of the m6A methylation machinery it associates with other proteins that determine m6A methyltransferase activity.
Biological function summary

This protein plays a significant part in mRNA modification by acting as an important component of the m6A writer complex. It contributes to the regulation of gene expression by modulating mRNA stability and translation. The m6A writer complex which includes METTL3 METTL14 and WTAP often coordinates with VIRMA to ensure proper mRNA methylation affecting cellular processes such as differentiation and proliferation. Its involvement in this complex implies its importance for mRNA metabolism and gene expression regulation.

Pathways

VIRMA significantly impacts the RNA processing and degradation pathways. Within these pathways it interacts with proteins such as METTL3 and METTL14 to influence mRNA splicing and degradation which are vital for cellular homeostasis. Moreover VIRMA is closely linked to the circadian rhythm pathway. It regulates mRNA targets affecting the rhythmic expression of clock genes underlining cellular timing mechanisms.

VIRMA is connected to cancer and metabolic disorders. Alterations in its methylation activity correlate with tumorigenesis and cancer progression particularly in liver cancer. It cooperates with proteins like METTL3 in promoting oncogenic mRNA translation leading to uncontrolled cell growth. Additionally dysregulation of RNA methylation involving VIRMA influences the metabolism contributing to disorders like obesity. This makes it an attractive target for therapeutic strategies aiming to modulate abnormal methylation patterns.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 24981863, PubMed : 29507755). Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs in the 3'-UTR near the stop codon : recruits the catalytic core components METTL3 and METTL14, thereby guiding m6A methylation at specific sites (PubMed : 29507755). Required for mRNA polyadenylation via its role in selective m6A methylation : m6A methylation of mRNAs in the 3'-UTR near the stop codon correlating with alternative polyadenylation (APA) (PubMed : 29507755).
See full target information VIRMA
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Product promise

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For full details, please see our Terms & Conditions

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