Anti-Visfatin antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody (AB24149)

Immunofluorescent analysis of 70% confluent log phase U87MG cells, 4% paraformaldehyde fixed and permeabilized with 0.1% Triton™ X-100, and blocked with 5% BSA for 1 hour at room temperature. ab24149 labeling Vistafin with 1 μg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-Visfatin antibody (AB24149)

Detection : chemiluminescence

All lanes:

Western blot - Anti-Visfatin antibody (ab24149) at 1/250 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 2:

PC3 (Human prostate adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

Lane 5:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

• WB

Unknown

Western blot - Anti-Visfatin antibody (AB24149)

All lanes:

Western blot - Anti-Visfatin antibody (ab24149)

All lanes:

Human adipose tissue lysate

Predicted band size: 55 kDa

Observed band size: 52 kDa

false