Rabbit Recombinant Monoclonal Visfatin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NAMPT aa 400-450.
Images
![Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (AB252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunoprecipitation-img88396.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (AB252558)")
![Western blot - Anti-Visfatin antibody [BLR058F] (AB252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--western-blot-img107887.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Visfatin antibody [BLR058F] (AB252558)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (AB252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88738.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (AB252558)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (AB252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118090.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (AB252558)")
![Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (AB252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunocytochemistry-immunofluorescence-img104334.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (AB252558)")
Publications
Filter by
- Journal of cachexia, sarcopenia and muscle 14:1003-10182023Nicotinamide Phosphoribosyltransferase-elevated NAD biosynthesis prevents muscle disuse atrophy by reversing mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Zhang,Yingming Wang,Shuai Lu,Rui Zhong,Zhilin Liu,Qichun Zhao,Chongyang WangPubMed 36864250
- Microbiology and immunology 67:129-1412023Silencing of circ-NT5C2 retards the progression of IL-1β-induced osteoarthritis in an in vitro cell model by targeting the miR-142-5p/NAMPT axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Yan-Dong Wang,Yong-Jun Xing,Peng-Jun Liu,Jian-Hui YangPubMed 36540014
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human NAMPT aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P43490
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 5 μl/1 mg lysate |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
Alternative names
PBEF, PBEF1, NAMPT, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Visfatin, Pre-B cell-enhancing factor
Recommended products
Rabbit Recombinant Monoclonal Visfatin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NAMPT aa 400-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human NAMPT aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P43490
- Clone number
- BLR058F
- Concentration
- Purification notes
Purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunoprecipitation-img88396.jpg "Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)")
Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)
Visfatin was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252558 used for IP at 5 μl per reaction. For WB 1/1000 dilution.Lane 1: ab252558 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293Twhole cell lysate.Chemiluminescence detection: 75 seconds.
All lanes: Immunoprecipitation - Anti-Visfatin antibody [BLR058F] (ab252558)
Predicted band size: 55 kDa
![Western blot - Anti-Visfatin antibody [BLR058F] (ab252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--western-blot-img107887.jpg "Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)")
Western blot - Anti-Visfatin antibody [BLR058F] (ab252558)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-Visfatin antibody [BLR058F] (ab252558) at 1/1000 dilution
Lane 1: RKO (Human cell line) whole cell lysate at 50 µg
Lane 2: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 50 µg
Lane 3: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 50 µg
Lane 4: GaMG (Human cell line) whole cell lysate at 50 µg
Lane 5: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 6: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 7: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg
Lane 8: LNCaP (Human prostate cancer cell line) whole cell lysate at 50 µg
Lane 9: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg
Lane 10: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 11: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 55 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88738.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118090.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Visfatin antibody [BLR058F] (ab252558)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Visfatin with ab252558 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
![Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558), expandable thumbnail](https://content.abcam.com/products/images/visfatin-antibody-blr058f-ab252558--immunocytochemistry-immunofluorescence-img104334.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558)")
Immunocytochemistry/ Immunofluorescence - Anti-Visfatin antibody [BLR058F] (ab252558)
FFPE section of NCI-H460 (Human lung cancer cell line) cells labeling Visfatin using ab252558 at a 1/250 dilution in ICC.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Substrate: DAB.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com