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AB214933

Anti-VISTA antibody

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(2 Publications)

Sheep Polyclonal VISTA antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Vsir aa 1-200.

View Alternative Names

Dies1, PD-1H, VISTA, Vsir, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor

1 Images
Western blot - Anti-VISTA antibody (AB214933)
  • WB

Supplier Data

Western blot - Anti-VISTA antibody (AB214933)

All lanes:

Western blot - Anti-VISTA antibody (ab214933) at 1/1000 dilution

All lanes:

Mouse embryonic tissue lysate

Predicted band size: 34 kDa

Observed band size: 30 kDa

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Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Vsir aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9D659

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The IgG fraction of immunized serum was purified by antigen affinity chromatography.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VISTA also known as V-domain Ig suppressor of T cell activation or Gi24 is a protein significant in immune regulation. It is a type I transmembrane glycoprotein with an apparent mass of approximately 55 kDa. VISTA is expressed on hematopoietic cells specifically within immune system components such as myeloid cells and T cells. Researchers have identified biotinylated chimeric proteins which facilitate the study of VISTA's expression and interactions in various immunological research models.
Biological function summary

VISTA functions in immune checkpoint regulation by modulating T cell activity. It acts as both a ligand and a receptor influencing immune responses. As part of an immune complex VISTA interacts with other checkpoint proteins to maintain immune homeostasis and prevent autoimmunity. Its role mirrors those of other B7 family members like PD-1 and CTLA-4 which are involved in negative immune regulation.

Pathways

VISTA integrates into the broader framework of immune signaling pathways. It contributes significantly to the immune checkpoint pathway similar to CTLA-4 and PD-1 which mediate immune cell proliferation and activation. By interacting with these proteins VISTA plays an important role in maintaining self-tolerance and regulating inflammatory responses functioning parallel to other proteins such as CD28 and B7-H5 in the immune checkpoint pathway.

VISTA's improper regulation associates with autoimmune diseases and cancer. Its expression influences the progression of conditions like rheumatoid arthritis where it modulates immune responses and inflammation. Additionally in oncology VISTA acts as an inhibitory molecule; hence targeting it can enhance anti-tumor immunity similar to therapies targeting proteins like PD-1 and PD-L1. Understanding VISTA's intricate connections helps in developing therapies for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Immunoregulatory receptor which inhibits the T-cell response (PubMed : 21383057, PubMed : 24743150, PubMed : 25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed : 20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).
See full target information Vsir
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 51:1326-1352 PubMed36718960

2023

Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksej Drino,Lisa König,Charlotte Capitanchik,Nasim Sanadgol,Eva Janisiw,Tom Rappol,Elisa Vilardo,Matthias R Schaefer

Cell reports 30:510-524.e6 PubMed31940493

2020

FOXD3 Regulates VISTA Expression in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sheera R Rosenbaum,Meghan Knecht,Mehri Mollaee,Zhijiu Zhong,Dan A Erkes,Peter A McCue,Inna Chervoneva,Adam C Berger,Jennifer A Lo,David E Fisher,Jeffrey E Gershenwald,Michael A Davies,Timothy J Purwin,Andrew E Aplin
View all publications
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