Sheep Polyclonal VISTA antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Vsir aa 1-200.
Images
Publications
Filter by
- Cell reports 30:510-524.e62020FOXD3 Regulates VISTA Expression in Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheera R Rosenbaum,Meghan Knecht,Mehri Mollaee,Zhijiu Zhong,Dan A Erkes,Peter A McCue,Inna Chervoneva,Adam C Berger,Jennifer A Lo,David E Fisher,Jeffrey E Gershenwald,Michael A Davies,Timothy J Purwin,Andrew E AplinPubMed 31940493
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Vsir aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9D659
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/2000 | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Immunoregulatory receptor which inhibits the T-cell response (PubMed:21383057, PubMed:24743150, PubMed:25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed:20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).
Alternative names
Dies1, PD-1H, VISTA, Vsir, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor
Recommended products
Sheep Polyclonal VISTA antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Vsir aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Vsir aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9D659
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The IgG fraction of immunized serum was purified by antigen affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VISTA also known as V-domain Ig suppressor of T cell activation or Gi24 is a protein significant in immune regulation. It is a type I transmembrane glycoprotein with an apparent mass of approximately 55 kDa. VISTA is expressed on hematopoietic cells specifically within immune system components such as myeloid cells and T cells. Researchers have identified biotinylated chimeric proteins which facilitate the study of VISTA's expression and interactions in various immunological research models.
- Biological function summary
VISTA functions in immune checkpoint regulation by modulating T cell activity. It acts as both a ligand and a receptor influencing immune responses. As part of an immune complex VISTA interacts with other checkpoint proteins to maintain immune homeostasis and prevent autoimmunity. Its role mirrors those of other B7 family members like PD-1 and CTLA-4 which are involved in negative immune regulation.
- Pathways
VISTA integrates into the broader framework of immune signaling pathways. It contributes significantly to the immune checkpoint pathway similar to CTLA-4 and PD-1 which mediate immune cell proliferation and activation. By interacting with these proteins VISTA plays an important role in maintaining self-tolerance and regulating inflammatory responses functioning parallel to other proteins such as CD28 and B7-H5 in the immune checkpoint pathway.
- Associated diseases and disorders
VISTA's improper regulation associates with autoimmune diseases and cancer. Its expression influences the progression of conditions like rheumatoid arthritis where it modulates immune responses and inflammation. Additionally in oncology VISTA acts as an inhibitory molecule; hence targeting it can enhance anti-tumor immunity similar to therapies targeting proteins like PD-1 and PD-L1. Understanding VISTA's intricate connections helps in developing therapies for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-VISTA antibody (ab214933)
All lanes: Western blot - Anti-VISTA antibody (ab214933) at 1/1000 dilution
All lanes: Mouse embryonic tissue lysate
Predicted band size: 34 kDa
Observed band size: 30 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com