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Knockout Tested Rabbit Recombinant Monoclonal VISTA antibody. Suitable for IHC-P, ICC, IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human VSIR aa 250 to C-terminus.

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Images

Immunoprecipitation - Anti-VISTA antibody [BLR035F] (AB243891), expandable thumbnail
  • Immunocytochemistry - Anti-VISTA antibody [BLR035F] (AB243891), expandable thumbnail
  • Western blot - Anti-VISTA antibody [BLR035F] (AB243891), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] (AB243891), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] (AB243891), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human VSIR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7M9

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCIPFlow CytWB
Human
Tested
Tested
Tested
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Use 20μl/mg lysate.

Expected
Expected

Species
Human
Dilution info
2 µL for 106 Cells
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).

Alternative names

C10orf54, SISP1, VISTA, PP2135, UNQ730/PRO1412, VSIR, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, Stress-induced secreted protein-1, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, Sisp-1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal VISTA antibody. Suitable for IHC-P, ICC, IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human VSIR aa 250 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human VSIR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7M9
Clone number
BLR035F
Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

VISTA also known as V-domain Ig suppressor of T cell activation or Gi24 is a protein significant in immune regulation. It is a type I transmembrane glycoprotein with an apparent mass of approximately 55 kDa. VISTA is expressed on hematopoietic cells specifically within immune system components such as myeloid cells and T cells. Researchers have identified biotinylated chimeric proteins which facilitate the study of VISTA's expression and interactions in various immunological research models.

Biological function summary

VISTA functions in immune checkpoint regulation by modulating T cell activity. It acts as both a ligand and a receptor influencing immune responses. As part of an immune complex VISTA interacts with other checkpoint proteins to maintain immune homeostasis and prevent autoimmunity. Its role mirrors those of other B7 family members like PD-1 and CTLA-4 which are involved in negative immune regulation.

Pathways

VISTA integrates into the broader framework of immune signaling pathways. It contributes significantly to the immune checkpoint pathway similar to CTLA-4 and PD-1 which mediate immune cell proliferation and activation. By interacting with these proteins VISTA plays an important role in maintaining self-tolerance and regulating inflammatory responses functioning parallel to other proteins such as CD28 and B7-H5 in the immune checkpoint pathway.

Associated diseases and disorders

VISTA's improper regulation associates with autoimmune diseases and cancer. Its expression influences the progression of conditions like rheumatoid arthritis where it modulates immune responses and inflammation. Additionally in oncology VISTA acts as an inhibitory molecule; hence targeting it can enhance anti-tumor immunity similar to therapies targeting proteins like PD-1 and PD-L1. Understanding VISTA's intricate connections helps in developing therapies for these diseases.

Product promise

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Full details and terms and conditions can be found here:
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6 product images

  • Immunoprecipitation - Anti-VISTA antibody [BLR035F] (ab243891), expandable thumbnail

    Immunoprecipitation - Anti-VISTA antibody [BLR035F] (ab243891)

    VISTA was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243891 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243891 at 1/1000 dilution. Lane 1: ab243891 IP in Jurkat whole cell lysate.

    Lane 2: Contol IgG in Jurkat whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

    All lanes: Immunoprecipitation - Anti-VISTA antibody [BLR035F] (ab243891)

    Predicted band size: 34 kDa

  • Immunocytochemistry - Anti-VISTA antibody [BLR035F] (ab243891), expandable thumbnail

    Immunocytochemistry - Anti-VISTA antibody [BLR035F] (ab243891)

    Formalin-fixed, paraffin-embedded NCI H226 cells labeling VISTA using ab243891 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

  • Western blot - Anti-VISTA antibody [BLR035F] (ab243891), expandable thumbnail

    Western blot - Anti-VISTA antibody [BLR035F] (ab243891)

    Western blot analysis using ab243891 at 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate (50 μg).

    Lane 2: MOLT-4 whole cell lysate (50 μg).

    Lane 3: MJ whole cell lysate (50 μg).

    Lane 4: RPMI 8226 whole cell lysate (50 μg).

    Lane 5: HeLa whole cell lysate (50 μg).

    Lane 6: KG-1 whole cell lysate (50 μg).

    Lane 7: HEK-293T whole cell lysate (50 μg).

    Lane 8: SUP-M2 whole cell lysate (50 μg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.

    All lanes: Western blot - Anti-VISTA antibody [BLR035F] (ab243891)

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] (ab243891), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] (ab243891)

    Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for VISTA using ab243891 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] (ab243891), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] (ab243891)

    Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling VISTA with ab243891 at a 1/250 dilution, followed by HRP-conjugated Goat Anti-Rabit IgG secondary antibody. Substrate: Opal. Counterstain: DAPI (blue).

  • Western blot - Anti-VISTA antibody [BLR035F] (ab243891), expandable thumbnail

    Western blot - Anti-VISTA antibody [BLR035F] (ab243891)

    Western blot: Anti-VSIR antibody [BLR035F] (ab243891) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab243891 was shown to bind specifically to VSIR. A band was observed at 50-60 kDa in wild-type A549 cell lysates with no signal observed at this size in VSIR knockout cell line. To generate this image, wild-type and VSIR knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-VISTA antibody [BLR035F] (ab243891) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: VSIR knockout A549 cell lysate at 20 µg

    Lane 3: DU 145 cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

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Product protocols

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