Anti-VISTA antibody [BLR035F] - BSA free

• Flow Cyt

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Flow Cytometry - Anti-VISTA antibody [BLR035F] - BSA free (AB272080)

Flow cytometric analysis of human peripheral blood mononuclear cells labelling VISTA with ab243891 (shaded) compared with isotype control (un-shaded). DyLight® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] - BSA free (AB272080)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for VISTA using ab243891 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] - BSA free (AB272080)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling VISTA with ab243891 at a 1/250 dilution, followed by HRP-conjugated Goat Anti-Rabit IgG secondary antibody. Substrate : Opal. Counterstain : DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).

• ICC

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Immunocytochemistry - Anti-VISTA antibody [BLR035F] - BSA free (AB272080)

Formalin-fixed, paraffin-embedded NCI H226 cells labeling VISTA using ab243891 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).

• IP

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Immunoprecipitation - Anti-VISTA antibody [BLR035F] - BSA free (AB272080)

VISTA was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243891 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using ab243891 at 1/1000 dilution.

Lane 1 : ab243891 IP in Jurkat whole cell lysate.

Lane 2 : Contol IgG in Jurkat whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).

All lanes:

Immunoprecipitation - Anti-VISTA antibody [BLR035F] (<a href='/en-us/products/primary-antibodies/vista-antibody-blr035f-ab243891'>ab243891</a>)

Predicted band size: 34 kDa

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• WB

Supplier Data

Western blot - Anti-VISTA antibody [BLR035F] - BSA free (AB272080)

Detection : chemiluminescence

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243891).

All lanes:

Western blot - Anti-VISTA antibody [BLR035F] - BSA free (ab272080) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg

Lane 3:

MJ whole cell lysate at 50 µg

Lane 4:

RPMI 8226 whole cell lysate at 50 µg

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 6:

KG-1 whole cell lysate at 50 µg

Lane 7:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 8:

Sup-M2 (Human) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 34 kDa

false

Exposure time: 30s