Anti-VISTA antibody [BLR035F] - BSA free - Carrier free (ab272080)

Anti-VISTA antibody [BLR035F] - BSA free (ab272080) is a rabbit monoclonal antibody that is used to detect VISTA in Western Blot, Flow Cytometry, IP, IHC-P. Suitable for Human samples.

- BSA free for reduced non-specific background staining

-Recombinant format for unrivalled batch-batch consistency

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Images

Immunoprecipitation - Anti-VISTA antibody [BLR035F] - BSA free (AB272080), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human VSIR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7M9

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC	IP	Flow Cyt	WB
Human	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

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Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information VSIR

Function

Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).

Alternative names

C10orf54, SISP1, VISTA, PP2135, UNQ730/PRO1412, VSIR, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, Stress-induced secreted protein-1, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor, Sisp-1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human VSIR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7M9
Clone number: BLR035F
Concentration
Purification notes: Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-VISTA antibody [BLR035F] - BSA free (ab272080) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

Other related products

We have a range of other formats of antibody clone [BLR035F] also available for your convenience:
ab243891, ab272080

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VISTA also known as V-domain Ig suppressor of T cell activation or Gi24 is a protein significant in immune regulation. It is a type I transmembrane glycoprotein with an apparent mass of approximately 55 kDa. VISTA is expressed on hematopoietic cells specifically within immune system components such as myeloid cells and T cells. Researchers have identified biotinylated chimeric proteins which facilitate the study of VISTA's expression and interactions in various immunological research models.

Biological function summary

VISTA functions in immune checkpoint regulation by modulating T cell activity. It acts as both a ligand and a receptor influencing immune responses. As part of an immune complex VISTA interacts with other checkpoint proteins to maintain immune homeostasis and prevent autoimmunity. Its role mirrors those of other B7 family members like PD-1 and CTLA-4 which are involved in negative immune regulation.

Pathways

VISTA integrates into the broader framework of immune signaling pathways. It contributes significantly to the immune checkpoint pathway similar to CTLA-4 and PD-1 which mediate immune cell proliferation and activation. By interacting with these proteins VISTA plays an important role in maintaining self-tolerance and regulating inflammatory responses functioning parallel to other proteins such as CD28 and B7-H5 in the immune checkpoint pathway.

Associated diseases and disorders

VISTA's improper regulation associates with autoimmune diseases and cancer. Its expression influences the progression of conditions like rheumatoid arthritis where it modulates immune responses and inflammation. Additionally in oncology VISTA acts as an inhibitory molecule; hence targeting it can enhance anti-tumor immunity similar to therapies targeting proteins like PD-1 and PD-L1. Understanding VISTA's intricate connections helps in developing therapies for these diseases.

Product promise

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6 product images

Immunoprecipitation - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)
VISTA was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with Anti-VISTA antibody [BLR035F] ab243891 at 20 μl per reaction. Western blot was performed on the immunoprecipitate using Anti-VISTA antibody [BLR035F] ab243891 at 1/1000 dilution.
Lane 1: Anti-VISTA antibody [BLR035F] ab243891 IP in Jurkat whole cell lysate.
Lane 2: Contol IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-VISTA antibody [BLR035F] ab243891).
All lanes: Immunoprecipitation - Anti-VISTA antibody [BLR035F] (Anti-VISTA antibody [BLR035F] ab243891)
Predicted band size: 34 kDa
Western blot - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)
Detection: chemiluminescence
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-VISTA antibody [BLR035F] ab243891).
All lanes: Western blot - Anti-VISTA antibody [BLR035F] - BSA free (ab272080) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg
Lane 3: MJ whole cell lysate at 50 µg
Lane 4: RPMI 8226 whole cell lysate at 50 µg
Lane 5: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 6: KG-1 whole cell lysate at 50 µg
Lane 7: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 8: Sup-M2 (Human) whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 34 kDa
Exposure time: 30s
Immunocytochemistry - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)
Formalin-fixed, paraffin-embedded NCI H226 cells labeling VISTA using Anti-VISTA antibody [BLR035F] ab243891 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-VISTA antibody [BLR035F] ab243891).
Flow Cytometry - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)
Flow cytometric analysis of human peripheral blood mononuclear cells labelling VISTA with Anti-VISTA antibody [BLR035F] ab243891 (shaded) compared with isotype control (un-shaded). DyLight® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-VISTA antibody [BLR035F] ab243891).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for VISTA using Anti-VISTA antibody [BLR035F] ab243891 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-VISTA antibody [BLR035F] ab243891).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] - BSA free (ab272080)
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling VISTA with Anti-VISTA antibody [BLR035F] ab243891 at a 1/250 dilution, followed by HRP-conjugated Goat Anti-Rabit IgG secondary antibody. Substrate: Opal. Counterstain: DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-VISTA antibody [BLR035F] ab243891).

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Anti-VISTA antibody [BLR035F] - BSA free

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