Rabbit Recombinant Monoclonal VISTA antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Images
![Sandwich ELISA - Anti-VISTA antibody [EPR20959-70] - BSA and Azide free (Detector) (AB244994), expandable thumbnail](https://content.abcam.com/products/images/vista-antibody-epr20959-70-bsa-and-azide-free-detector-ab244994--sandwich-elisa-img51836.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-VISTA antibody [EPR20959-70] - BSA and Azide free (Detector) (AB244994)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Immunoregulatory receptor which inhibits the T-cell response (PubMed:21383057, PubMed:24743150, PubMed:25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed:20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).
Alternative names
Dies1, PD-1H, VISTA, Vsir, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor
Recommended products
Rabbit Recombinant Monoclonal VISTA antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20959-70
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab244994 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse PD-1H. The recommended pair for sandwich ELISA is:
Capture: Anti-VISTA antibody [EPR20959-74] - BSA and Azide free (Capture) ab244727, Mouse PD-1H Capture Antibody (unconjugated)
Detector: ab244994, Mouse PD-1H Detector Antibody (unconjugated)
The reference range value is 390.63 - 25000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VISTA also known as V-domain Ig suppressor of T cell activation or Gi24 is a protein significant in immune regulation. It is a type I transmembrane glycoprotein with an apparent mass of approximately 55 kDa. VISTA is expressed on hematopoietic cells specifically within immune system components such as myeloid cells and T cells. Researchers have identified biotinylated chimeric proteins which facilitate the study of VISTA's expression and interactions in various immunological research models.
- Biological function summary
VISTA functions in immune checkpoint regulation by modulating T cell activity. It acts as both a ligand and a receptor influencing immune responses. As part of an immune complex VISTA interacts with other checkpoint proteins to maintain immune homeostasis and prevent autoimmunity. Its role mirrors those of other B7 family members like PD-1 and CTLA-4 which are involved in negative immune regulation.
- Pathways
VISTA integrates into the broader framework of immune signaling pathways. It contributes significantly to the immune checkpoint pathway similar to CTLA-4 and PD-1 which mediate immune cell proliferation and activation. By interacting with these proteins VISTA plays an important role in maintaining self-tolerance and regulating inflammatory responses functioning parallel to other proteins such as CD28 and B7-H5 in the immune checkpoint pathway.
- Associated diseases and disorders
VISTA's improper regulation associates with autoimmune diseases and cancer. Its expression influences the progression of conditions like rheumatoid arthritis where it modulates immune responses and inflammation. Additionally in oncology VISTA acts as an inhibitory molecule; hence targeting it can enhance anti-tumor immunity similar to therapies targeting proteins like PD-1 and PD-L1. Understanding VISTA's intricate connections helps in developing therapies for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-VISTA antibody [EPR20959-70] - BSA and Azide free (Detector) (ab244994), expandable thumbnail](https://content.abcam.com/products/images/vista-antibody-epr20959-70-bsa-and-azide-free-detector-ab244994--sandwich-elisa-img51836.jpg "Sandwich ELISA - Anti-VISTA antibody [EPR20959-70] - BSA and Azide free (Detector) (ab244994)")
Sandwich ELISA - Anti-VISTA antibody [EPR20959-70] - BSA and Azide free (Detector) (ab244994)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse PD-1H ELISA Kit ab231933).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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