Rabbit Recombinant Monoclonal Vitamin B2 antibody. Suitable for Dot and reacts with Synthetic peptide samples.
Images
![Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] (AB218125), expandable thumbnail](https://content.abcam.com/products/images/vitamin-b2-antibody-epr20853-141-ab218125--dot-blot-img3073.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] (AB218125)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | |
|---|---|
| Synthetic peptide | Tested |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
Target data
Alternative names
FMN, Flavin Mononucleotide, Riboflavin
Recommended products
Rabbit Recombinant Monoclonal Vitamin B2 antibody. Suitable for Dot and reacts with Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20853-141
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] (ab218125), expandable thumbnail](https://content.abcam.com/products/images/vitamin-b2-antibody-epr20853-141-ab218125--dot-blot-img3073.jpg "Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)")
Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] (ab218125)
Dot blot analysis of Vitamin B12 using ab218125 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.
Lane 1: Ovalbumin (OVA)-Riboflavin
Lane 2: OVA-Folic acid (carboxyl conjugation)
Lane 3: OVA-Folic acid (amino conjugation)
Lane 4: OVA-Histamine
Exposure time: 3 minutes.
Blocking/Diluting buffer: 5% NFDM/TBST
Folic acid and Histamine are the analogue of Riboflavin.
Ovalbumin (OVA)-conjugated small molecules are kindly provided by Dr Rao Yi.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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