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AB254211

Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free

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Rabbit Recombinant Monoclonal Vitamin B2 antibody. Carrier free. Suitable for Dot and reacts with Synthetic peptide samples.

View Alternative Names

FMN, Flavin Mononucleotide, Riboflavin

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Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free (AB254211)
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Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free (AB254211)

Dot blot analysis of Vitamin B12 using ab218125 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1 : Ovalbumin (OVA)-Riboflavin

Lane 2 : OVA-Folic acid (carboxyl conjugation)

Lane 3 : OVA-Folic acid (amino conjugation)

Lane 4 : OVA-Histamine

Exposure time : 3 minutes.

Blocking/Diluting buffer : 5% NFDM/TBST

Folic acid and Histamine are the analogue of Riboflavin.

Ovalbumin (OVA)-conjugated small molecules are kindly provided by Dr Rao Yi.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218125).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20853-141

Isotype

IgG

Carrier free

Yes

Applications

Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
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Product details

ab254211 is the carrier-free version of ab218125.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Vitamin B2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com