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AB23485

Anti-Vitamin D Binding protein antibody [19E8]

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(2 Publications)

Anti-Vitamin D Binding protein antibody [19E8] (ab23485) is a mouse monoclonal antibody detecting Vitamin D Binding protein in IHC-P, ELISA. Suitable for Human.

- Trusted since 2005

View Alternative Names

Vitamin D-binding protein, DBP, VDB, Gc protein-derived macrophage activating factor, Gc-globulin, Group-specific component, Vitamin D-binding protein-macrophage activating factor, Gc-MAF, GcMAF, Gc, DBP-maf, GC

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (AB23485)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [19E8] (AB23485)

IHC image of ab23485 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23485, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

19E8

Isotype

IgG2

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human GC.

P02774

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Vitamin D Binding protein antibody [19E8] (ab23485) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), ELISA in Human samples.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 2.9% Sodium chloride, 0.0268% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
See full target information GC
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:105509 PubMed38042493

2023

N-acetyl cysteine turns EPAC activators into potent killers of acute lymphoblastic leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nina Richartz,Wojciech Pietka,Ajay Yadav,Monica Bostad,Sampada Bhagwat,Soheil Naderi,Elin Hallan Naderi,Trond Stokke,Ellen Ruud,Heidi Kiil Blomhoff

Diabetes 65:1341-9 PubMed26983959

2016

Expression-Based Genome-Wide Association Study Links Vitamin D-Binding Protein With Autoantigenicity in Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Kodama,Zhiyuan Zhao,Kyoko Toda,Linda Yip,Rebecca Fuhlbrigge,Dongmei Miao,C Garrison Fathman,Satoru Yamada,Atul J Butte,Liping Yu
View all publications
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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