AB81307

Anti-Vitamin D Binding protein antibody [EP2923Y]

Name: Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) | Abcam
Brand: Abcam Limited
SKU: AB81307
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal Vitamin D Binding protein antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Vitamin D-binding protein, DBP, VDB, Gc protein-derived macrophage activating factor, Gc-globulin, Group-specific component, Vitamin D-binding protein-macrophage activating factor, Gc-MAF, GcMAF, Gc, DBP-maf, GC

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (AB81307)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (AB81307)

Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Lab

Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (AB81307)

Blocking and Diluting buffer 5% NFDM /TBST

All lanes:

Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 52 kDa

false

Exposure time: 3min

Carrier free

Anti-Vitamin D Binding protein antibody [EP2923Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2923Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.

See full target information GC

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 9:829490 PubMed35757319

2022

Vitamin D Binding Protein and Renal Injury in Acute Decompensated Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Diaz-Riera,Maisa García-Arguinzonis,Laura López,Xavier Garcia-Moll,Lina Badimon,Teresa Padró

Military Medical Research 9:4 PubMed35057868

2022

Potential roles of vitamin D binding protein in attenuating liver injury in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Xiao,Du-Chao Zhang,Ye Hu,Li-Cheng Song,Jian-Qiao Xu,Wan-Xue He,Pan Pan,Yu-Wei Wang,Li-Xin Xie

Oncology letters 19:3815-3826 PubMed32391095

2020

Identification of potential cervical cancer serum biomarkers in Thai patients.

Applications

Unspecified application

Species

Unspecified reactive species

Siriporn Keeratichamroen,Pantipa Subhasitanont,Daranee Chokchaichamnankit,Churat Weeraphan,Kittirat Saharat,Narongrit Sritana,Nuttavut Kantathavorn,Kriangpol Wiriyaukaradecha,Thaniya Sricharunrat,N Monique Paricharttanakul,Chirayu Auewarakul,Jisnuson Svasti,Chantragan Srisomsap

Fetal diagnosis and therapy 47:497-506 PubMed32097912

2020

First Trimester Protein Biomarkers for Risk of Spontaneous Preterm Birth: Identifying a Critical Need for More Rigorous Approaches to Biomarker Identification and Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Arlene M D'Silva,Jon A Hyett,Jens R Coorssen

Proteomics. Clinical applications 12:e1700088 PubMed29412517

2018

Plasma Proteomic Signatures in Early Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Baralla,Alessandro G Fois,Elisabetta Sotgiu,Elisabetta Zinellu,Arduino A Mangoni,Salvatore Sotgia,Angelo Zinellu,Pietro Pirina,Ciriaco Carru

Translational psychiatry 8:61 PubMed29531242

2018

Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein.

Applications

Unspecified application

Species

Unspecified reactive species

Brawnie Petrov,Ayat Aldoori,Cindy James,Kefeng Yang,Guillermo Perez Algorta,Aejin Lee,Liwen Zhang,Tao Lin,Reem Al Awadhi,Jonathan R Parquette,Arpad Samogyi,L Eugene Arnold,Mary A Fristad,Barbara Gracious,Ouliana Ziouzenkova

EMBO reports 17:178-87 PubMed26691212

2015

Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Li,Johanna Klughammer,Matthias Farlik,Thomas Penz,Andreas Spittler,Charlotte Barbieux,Ekaterine Berishvili,Christoph Bock,Stefan Kubicek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Vitamin D Binding protein antibody [EP2923Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Binding protein antibody [EP2923Y] (AB81307)

Western blot - Anti-Vitamin D Binding protein antibody [EP2923Y] (AB81307)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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