Rabbit Polyclonal Vitamin D Receptor antibody. Suitable for IP, EMSA, ChIP, WB, IHC-P, ICC/IF, ChIP-seq, IHC-Fr, Gel Shift Assay and reacts with Mouse, Rat, Chicken, Human samples. Cited in 73 publications. Immunogen corresponding to Synthetic Peptide within Human VDR aa 350 to C-terminus.
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- iScience 26:1076092023MYCT1 attenuates renal fibrosis and tubular injury in diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li,Qiu-Ling Fan,Tian-Kui Ma,Cong Liu,Hang Shi,Yuan-Yuan Sun,Yue Wang,Dong-Xue Ding,Ao Tang,Yu Qin,Qi Yang,Hong Ding,Hang-Yu Li,Wei-Neng FuPubMed 37664593
- Scientific reports 13:110882023VDR alleviates endothelial cell injury in arteriovenous fistula through inhibition of P66Shc-mediated mitochondrial ROS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Chun Han,Yu-Ting Liu,Hao Zhang,Yong Xu,Jun Liu,Hong Chen,Na Song,Dong-Lu Qin,Shikun YangPubMed 37422508
- International journal of molecular sciences 24:2023Exploiting Vitamin D Receptor and Its Ligands to Target Squamous Cell Carcinomas of the Head and Neck.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Koll,Désirée Gül,Manal I Elnouaem,Hanaa Raslan,Omneya R Ramadan,Shirley K Knauer,Sebastian Strieth,Jan Hagemann,Roland H Stauber,Aya KhamisPubMed 36902107
- Investigative ophthalmology & visual science 64:22023Calcipotriol Attenuates Form Deprivation Myopia Through a Signaling Pathway Parallel to TGF-β2-Induced Increases in Collagen Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiming Jiao,Peter Sol Reinach,Chengjie Huang,Lan Yu,Huiman Zhuang,Hongli Ran,Fei Zhao,Nethrajeith Srinivasalu,Jia Qu,Xiangtian ZhouPubMed 36723926
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:427-4422023Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH) D Deficiency and Downregulates GATA4-Dependent Rela Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuyi Wang,Jingyu Zhao,Haiyun Chen,Jiawen Zhou,Ao Chen,Jin'ge Zhang,Yue Wang,Zhiyuan Mao,Jiachen Wang,Xuehan Qiu,Yutong Chen,Rong Wang,Yongjie Zhang,Dengshun Miao,Jianliang JinPubMed 36625422
- International journal of biological sciences 19:610-62420231,25-Dihydroxyvitamin D Deficiency Accelerates Aging-related Osteoarthritis via Downregulation of Sirt1 in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Chen,Jiao Zhang,Jie Li,Ran Qin,Na Lu,David Goltzman,Dengshun Miao,Renlei YangPubMed 36632467
- Cancers 14:2022The Vitamin D Receptor-BIM Axis Overcomes Cisplatin Resistance in Head and Neck Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAya Khamis,Désirée Gül,Madita Wandrey,Qiang Lu,Shirley K Knauer,Christoph Reinhardt,Sebastian Strieth,Jan Hagemann,Roland H StauberPubMed 36291915
- Aging cell 21:e136802022Sirt1 overexpression improves senescence-associated pulmonary fibrosis induced by vitamin D deficiency through downregulating IL-11 transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiawen Zhou,Haiyun Chen,Qiuyi Wang,Sihan Chen,Rong Wang,Ziyang Wang,Cuicui Yang,Ao Chen,Jingyu Zhao,Zihao Zhou,Zhiyuan Mao,Guoping Zuo,Dengshun Miao,Jianliang JinPubMed 35906886
- Cell & bioscience 12:982022DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaochen Guan,Nan Zhu,Gang Tang,Yi Du,Ling Wang,Weijie YuanPubMed 35765066
- Molecular cancer 21:1322022Comprehensive characterization of the prostate tumor microenvironment identifies CXCR4/CXCL12 crosstalk as a novel antiangiogenic therapeutic target in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabel Heidegger,Georgios Fotakis,Anne Offermann,Jermaine Goveia,Sophia Daum,Stefan Salcher,Asma Noureen,Hetty Timmer-Bosscha,Georg Schäfer,Annemiek Walenkamp,Sven Perner,Aleksandar Beatovic,Matthieu Moisse,Christina Plattner,Anne Krogsdam,Johannes Haybaeck,Sieghart Sopper,Stefanie Thaler,Markus A Keller,Helmut Klocker,Zlatko Trajanoski,Dominik Wolf,Andreas PircherPubMed 35717322
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human VDR aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11473
Reactivity data
Select an application| IP | EMSA | ChIP | WB | IHC-P | ICC/IF | ChIP-seq | IHC-Fr | Gel Shift Assay | |
|---|---|---|---|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Expected |
| Mouse | Expected | Expected | Expected | Expected | Predicted | Expected | Expected | Expected | Expected |
| Rat | Expected | Expected | Expected | Expected | Predicted | Expected | Expected | Expected | Expected |
| Chicken | Expected | Expected | Expected | Expected | Predicted | Expected | Expected | Expected | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Saguinus oedipus | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
EMSA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
Species Rat | Dilution info - | Notes 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
Species Chicken | Dilution info - | Notes 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
Species Human | Dilution info - | Notes 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/4000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus, Rat, Chicken, Mouse | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
ChIP-seq
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
Gel Shift Assay
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Saguinus oedipus | Dilution info - | Notes - |
Associated Products
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Target data
Function
Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells (PubMed:10678179, PubMed:15728261, PubMed:16913708, PubMed:28698609, PubMed:37478846). Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR (PubMed:28698609). The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes (PubMed:28698609). Plays a central role in calcium homeostasis (By similarity). Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites (PubMed:12016314, PubMed:32354638).
Alternative names
NR1I1, VDR, Vitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1
Recommended products
Rabbit Polyclonal Vitamin D Receptor antibody. Suitable for IP, EMSA, ChIP, WB, IHC-P, ICC/IF, ChIP-seq, IHC-Fr, Gel Shift Assay and reacts with Mouse, Rat, Chicken, Human samples. Cited in 73 publications. Immunogen corresponding to Synthetic Peptide within Human VDR aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human VDR aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11473
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)
ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
Left panel: with primary antibody at 1/2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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