JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229821

Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Cholecalciferol antibody. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

1 25 dihydroxyvitamin D3 receptor, 25-dihydroxyvitamin D3 receptor, GLRA1_HUMAN, Glycine receptor 48 kDa subunit, Glycine receptor alpha 1, Glycine receptor strychnine-binding subunit, Glycine receptor subunit alpha-1, Glycine receptor, alpha 1 subunit, HKPX1, Member 1, NR1I1, Nuclear receptor subfamily 1 group I member 1, PPP1R163, Protein phosphatase 1, regulatory subunit 163, STHE, VDR_HUMAN, Vitamin D (1,25- dihydroxyvitamin D3) receptor, Vitamin D hormone receptor, Vitamin D nuclear receptor variant 1, Vitamin D receptor, Vitamin D3 receptor

2 Images
Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (AB229821)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (AB229821)

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821).

Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 30-35 ng/mL of ab229821 (50 μL) for 30 minutes by shaking.

This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (AB229821)
  • cELISA

Supplier Data

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (AB229821)

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] (ab229821) used at 8.3 ng/mL.

The results show cross-reactivity with Vitamin D2.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R6-74-3

Isotype

IgG

Carrier free

No

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "30-35 ng/mL", "cELISA-species-notes": "<p>Use at 30-35 ng/mL.</p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Cholecalciferol
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:911715 PubMed35795047

2022

CircASPH Promotes Hepatocellular Carcinoma Progression Through Methylation and Expression of HAO2.

Applications

Unspecified application

Species

Unspecified reactive species

Han Zhuo,Jinguo Xia,Jin Zhang,Junwei Tang,Sheng Han,Qitong Zheng,Deming Zhu,Feihong Zhang,Zhenggang Xu,Dongwei Sun,Zhongming Tan,Chen Wu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB229822

Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12]

RabMAb|Recombinant

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (AB229822)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB229822

Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12]

RabMAb|Recombinant

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-6-271-12] (AB229822)

  • 0
  • 0
Primary Antibodies

AB215529

Anti-Testosterone antibody [EPPTX-R4]

RabMAb|Recombinant

Competitive ELISA - Anti-Testosterone antibody [EPPTX-R4] (AB215529)

  • 0
  • 0
Primary Antibodies

AB236471

Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free

RabMAb|Recombinant

Competitive ELISA - Anti-Vitamin D2+Vitamin D3 antibody [EPPTX-R6-74-3] - BSA and Azide free (AB236471)

  • 0
  • 0
Primary Antibodies

AB229820

Anti-Vitamin D3 antibody [EPPTX-R6-42-12]

RabMAb|Recombinant

Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (AB229820)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com